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Literature summary for 1.3.1.27 extracted from

  • Miyamaura, N.; Matsui, H.; Tahara, S.; Mizutani, J.; Chiba, S.
    Purification and some properties of 2-alkenal reductase of Muco griseo-cyanus AHU6044 (1984), Agric. Biol. Chem., 48, 185-192.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, complete inhibition Mucor circinelloides
Ca2+ 50 mM, 40% inhibition Mucor circinelloides
Hg2+ 1 mM, 86% inhibition Mucor circinelloides
N-ethylmaleimide 1 mM, 60% inhibition Mucor circinelloides
p-chloromercuribenzoate 1 mM, 90% inhibition Mucor circinelloides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
gel filtration Mucor circinelloides

Organism

Organism UniProt Comment Textmining
Mucor circinelloides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Mucor circinelloides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-propenal + NAD(P)H
-
Mucor circinelloides propanal + NAD(P)+
-
?
2-trans,4-trans-hexadienal + NAD(P)H i.e. sorbaldehyde, low reaction rate Mucor circinelloides 4-trans-hexenal + NAD(P)+
-
?
cis-2-heptenal + NAD(P)H
-
Mucor circinelloides heptanal + NAD(P)+
-
?
cis-2-octenal + NAD(P)H best substrate Mucor circinelloides octanal + NAD(P)+
-
?
trans-2-butenal + NAD(P)H very low reaction rate Mucor circinelloides butanal + NAD(P)+
-
?
trans-2-cinnamaldehyde + NAD(P)H low reaction rate Mucor circinelloides cinnamaldehyde + NAD(P)+
-
?
trans-2-decenal + NAD(P)H low reaction rate Mucor circinelloides decanal + NAD(P)+
-
?
trans-2-dodecenal + NAD(P)H low reaction rate Mucor circinelloides dodecanal + NAD(P)+
-
?
trans-2-heptenal + NAD(P)H low reaction rate Mucor circinelloides heptanal + NAD(P)+
-
?
trans-2-hexenal + NAD(P)H low reaction rate Mucor circinelloides hexanal + NAD(P)+
-
?
trans-2-nonenal + NAD(P)H + H+ low reaction rate Mucor circinelloides nonanal + NAD(P)+
-
?
trans-2-octenal + NAD(P)H
-
Mucor circinelloides octanal + NAD(P)+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 26000, SDS-disc gel electrophoresis Mucor circinelloides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Mucor circinelloides

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 labile below pH 4.0 Mucor circinelloides

Cofactor

Cofactor Comment Organism Structure
flavin enzyme is assumed to be a flavoprotein, prosthetic group may be FAD or FMN Mucor circinelloides
NADH
-
Mucor circinelloides
NADPH enzyme shows specificity for both NADPH and NADH Mucor circinelloides