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Literature summary for 1.3.1.24 extracted from

  • McCoubrey, W.K., Jr.; Maines, M.D.
    Site-directed mutagenesis of cysteine residues in biliverdin reductase. Roles in substrate and cofactor binding (1994), Eur. J. Biochem., 222, 597-603.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
biliverdin reductase cDNA cloned into pUC18, used for expression and purification Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
C280A although modification of either of the two cysteines located near the C-terminus significantly reduces activity with both cofactors, these mutations do not inactivate the enzyme, mutation of both C-terminal cysteines causes inactivation of the enzyme, comparison of Km values suggests that Cys 280 principally functions in substrate binding Rattus norvegicus
C291A although modification of either of the two cysteines located near the C-terminus significantly reduces activity with both cofactors, these mutations do not inactivate the enzyme, mutation of both C-terminal cysteines causes inactivation of the enzyme, comparison of Km values suggests that Cys 291 is predominantly involved in cofactor binding. Rattus norvegicus
C73A the modification of the amino-proximal cysteine, which is flanked by a tyrosine residue, completely inactivates the enzyme with NADH at pH 6.75 and NADPH at pH 8.7 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
NADPH in the presence of 0.005 mM biliverdin, biliverdin reductase C280A Rattus norvegicus
0.0029
-
NADPH in the presence of 0.005 mM biliverdin, biliverdin reductase wild-type Rattus norvegicus
0.0032
-
Biliverdin cosubstrate NADPH, biliverdin reductase C291A Rattus norvegicus
0.0034
-
Biliverdin cosubstrate NADPH, biliverdin reductase wild-type Rattus norvegicus
0.0049
-
Biliverdin cosubstrate NADH, biliverdin reductase wild-type Rattus norvegicus
0.0052
-
Biliverdin cosubstrate NADH, biliverdin reductase C291A Rattus norvegicus
0.0097
-
Biliverdin cosubstrate NADH, biliverdin reductase C280A Rattus norvegicus
0.0102
-
Biliverdin cosubstrate NADPH, biliverdin reductase C280A Rattus norvegicus
0.0133
-
NADPH in the presence of 0.005 mM biliverdin, biliverdin reductase C291A Rattus norvegicus
0.333
-
NADH in the presence of 0.005 mM biliverdin, biliverdin reductase wild-type Rattus norvegicus
0.394
-
NADH in the presence of 0.005 mM biliverdin, biliverdin reductase C280A Rattus norvegicus
0.567
-
NADH in the presence of 0.005 mM biliverdin, biliverdin reductase C291A Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of biliverdin reductase expressed in Escherichia coli and from rat tissue Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bilirubin + NAD+
-
Rattus norvegicus biliverdin + NADH + H+
-
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