Activating Compound | Comment | Organism | Structure |
---|---|---|---|
albumin | - |
Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Arsenobenzoate | 1 mM, slight | Sus scrofa | |
Biliverdin | - |
Rattus norvegicus | |
Biliverdin | substrate inhibition | Sus scrofa | |
FAD | - |
Sus scrofa | |
FMN | - |
Sus scrofa | |
iodoacetamide | - |
Rattus norvegicus | |
iodoacetamide | 1 mM, slight | Sus scrofa | |
N-ethylmaleimide | - |
Rattus norvegicus | |
N-ethylmaleimide | 1.0 mM | Sus scrofa | |
p-chloromercuribenzoate | - |
Rattus norvegicus | |
p-chloromercuribenzoate | 0.025 mM | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | 0.002 | Biliverdin | cosubstrate NADH | Sus scrofa | |
0.003 | - |
Biliverdin | cosubstrate NADPH, rat liver | Sus scrofa | |
0.003 | 0.005 | NADPH | pig spleen | Sus scrofa | |
1.5 | 2 | NADH | pig spleen, rat liver | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
gel filtration | Sus scrofa |
34000 | - |
1 * 34000, multiple forms, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
biliverdin + NAD(P)H | Sus scrofa | importance in linear tetrapyrrole metabolism | bilirubin + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spleen | - |
Rattus norvegicus | - |
spleen | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-20°C, several months -25°C, 1 month: no loss of activity, 2 months: 50% loss of activity | Rattus norvegicus |
-20°C, several months -25°C, 1 month: no loss of activity, 2 months: 50% loss of activity | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
biliverdin + NAD(P)H | - |
Rattus norvegicus | bilirubin + NAD(P)+ | - |
? | |
biliverdin + NAD(P)H | pig spleen: IXalpha-biliverdin: most effective substrate among the 4 biliverdin isomers | Sus scrofa | bilirubin + NAD(P)+ | - |
? | |
biliverdin + NAD(P)H | not: IXalpha-biliverdin methylester (pig spleen) | Sus scrofa | bilirubin + NAD(P)+ | - |
? | |
biliverdin + NAD(P)H | pig spleen: strictly specific to biliverdin, no other oxidoreductase activities | Sus scrofa | bilirubin + NAD(P)+ | - |
? | |
biliverdin + NAD(P)H | importance in linear tetrapyrrole metabolism | Sus scrofa | bilirubin + NAD(P)+ | - |
? | |
biliverdin + NADH + H+ | - |
Sus scrofa | bilirubin + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 34000, multiple forms, SDS-PAGE | Rattus norvegicus |
monomer | 1 * 34000, multiple forms, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
biliverdin + NADH | Sus scrofa |
8.5 | - |
biliverdin + NADPH | Rattus norvegicus |
8.5 | - |
biliverdin + NADPH | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Rattus norvegicus | |
NADH | - |
Sus scrofa | |
NADPH | - |
Rattus norvegicus | |
NADPH | - |
Sus scrofa |