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Literature summary for 1.3.1.22 extracted from
- 5alpha-Reductase activity in Lycopersicon esculentum: cloning and functional characterization of LeDET2 and evidence of the presence of two isoenzymes (2005), J. Steroid Biochem. Mol. Biol., 96, 287-299.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | two isoenzymes of 5alphaR is probably characteristic of the whole plant kingdom | Arabidopsis thaliana |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into vector pTarget and expressed in COS-7 cells | Arabidopsis thaliana |
| into vector pTarget and expressed in COS-7 cells | Solanum lycopersicum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K204E | det2-1 mutant, substitution of lysine for Glu-204 totally inactivates the steroid 5alpha-reductase activity, residual activity for campestenone and progesterone | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-MA | active at 0.01 mM | Solanum lycopersicum |  |
| AFA131 | active at 0.01 mM | Solanum lycopersicum | |
| AFA192 | active at 0.01 mM | Solanum lycopersicum | |
| AFA27 | active at 0.01 mM | Solanum lycopersicum | |
| AFA76 | active at 0.01 mM, most potent inhibitor among non-steroid compounds tested | Solanum lycopersicum | |
| AS601811 | active at 0.01 mM | Solanum lycopersicum | |
| finasteride | active at 0.01 mM | Solanum lycopersicum | |
| PD17 | active at 0.01 mM | Solanum lycopersicum | |
| PD91 | active at 0.01 mM | Solanum lycopersicum | |
| VG106 | active at 0.01 mM | Solanum lycopersicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00075 | - |
progesterone | - |
Solanum lycopersicum | |
| 0.02634 | - |
androstenedione | - |
Solanum lycopersicum | |
| 0.0446 | - |
testosterone | - |
Solanum lycopersicum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Solanum lycopersicum | Q5K2N1 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| callus | - |
Solanum lycopersicum | - |
| leaf | - |
Arabidopsis thaliana | - |
| leaf | higher levels | Solanum lycopersicum | - |
| root | - |
Solanum lycopersicum | - |
| seed | - |
Arabidopsis thaliana | - |
| seed | - |
Solanum lycopersicum | - |
| stem | - |
Solanum lycopersicum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| androstenedione + NADPH + H+ | - |
Solanum lycopersicum | 5alpha-androstan-3,17-dione + NADP+ | - |
? | |
| campestenone + NADPH | reduction at very low levels | Solanum lycopersicum | 5alpha-campestan-3-one + NADP+ | - |
? | |
| additional information | no activity for campestenone | Arabidopsis thaliana | ? | - |
? | |
| progesterone + NADPH + H+ | - |
Arabidopsis thaliana | 5alpha-pregnan-3-20-dione + NADP+ | - |
? | |
| progesterone + NADPH + H+ | - |
Solanum lycopersicum | 5alpha-pregnan-3-20-dione + NADP+ | - |
? | |
| testosterone + NADPH + H+ | - |
Solanum lycopersicum | 5alpha-dihydrotestosterone + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5alphaR | - |
Arabidopsis thaliana |
| 5alphaR | - |
Solanum lycopersicum |
| AtDET2 | - |
Arabidopsis thaliana |
| LeDET2 | - |
Solanum lycopersicum |
| steroid 5alpha-reductase | - |
Arabidopsis thaliana |
| steroid 5alpha-reductase | - |
Solanum lycopersicum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Solanum lycopersicum | |
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