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Literature summary for 1.3.1.21 extracted from

  • Lecain, E.; Chenivesse, X.; Spagnoli, R.; Pompon, D.
    Cloning by metabolic interference in yeast and enzymic characterization of Arabidopsis thaliana sterol DELTA7-reductase (1996), J. Biol. Chem., 271, 10866-10873.
    View publication on PubMed
Search for more literature for 1.3.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expression of cDNA in Saccharomyces cerevisiae and overexpression of recombinant protein Escherichia coli, sequence analysis Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol low activity Arabidopsis thaliana 5829
-
microsome
-
 Arabidopsis thaliana
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesta-5,7-dien-3-beta-ol + NADPH Arabidopsis thaliana highly regiospecific for DELTA7-bond reduction, but wide substrate specificity cholesterol + NADP+
-
 ?
ergosta-5,7-diene-3beta-ol + NADPH Arabidopsis thaliana also ergosta-5,24(28)-, ergosta-5,22,24(28)-eneols and cholesta-5,24- or cholesta-5,22,24-eneols can be formed in vivo in the mutants of Saccharomyces cerevisiae ergosta-5-ene-3beta-ol + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9LDU6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesta-5,7-dien-3-beta-ol + NADPH highly regiospecific for DELTA7-bond reduction, but wide substrate specificity Arabidopsis thaliana cholesterol + NADP+
-
 ?
cholesta-5,7-dien-3beta-ol + NADPH wide substrate spectrum Arabidopsis thaliana cholesterol + NADP+
-
 ?
ergosta-5,7,22-trien-3beta-ol + NADPH i.e. ergosterol Arabidopsis thaliana ergosta-5,22-diene-3beta-ol + NADP+
-
 ?
ergosta-5,7-diene-3beta-ol + NADPH
-
 Arabidopsis thaliana ergosta-5-eneol + NADP+
-
 ?
ergosta-5,7-diene-3beta-ol + NADPH also ergosta-5,24(28)-, ergosta-5,22,24(28)-eneols and cholesta-5,24- or cholesta-5,22,24-eneols can be formed in vivo in the mutants of Saccharomyces cerevisiae Arabidopsis thaliana ergosta-5-ene-3beta-ol + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
sterol DELTA7-reductase
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADPH no activity with NADH Arabidopsis thaliana