Literature summary for 1.3.1.2 extracted from

Dobritzsch, D.; Ricagno, S.; Schneider, G.; Schnackerz, K.D.; Lindqvist, Y.
Crystal structure of the productive ternary complex of dihydropyrimidine dehydrogenase with NADPH and 5-iodouracil. Implications for mechanism of inhibition and electron transfer (2002), J. Biol. Chem., 277, 13155-13166.

Search for more literature for 1.3.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Sus scrofa

Crystallization (Commentary)

Crystallization (Comment)	Organism
cocrystallization with 5-iodouracil 1 mM and NADPH 5 mM, ternary complex in 100 mM HEPES, pH 7.5, 22% polyethylene glycol 6000, uracil-4-acetic acid 1 mM, structure determination at different pH-values	Sus scrofa

Inhibitors

Inhibitors	Comment	Organism	Structure
5-Iodouracil	inhibition mechanism	Sus scrofa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Sus scrofa	5737	-

Organism

Organism	UniProt	Comment	Textmining
Sus scrofa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Sus scrofa

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Sus scrofa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
thymine + NADPH	-	Sus scrofa	dihydrothymine + NADP+	-	?
uracil + NADPH	-	Sus scrofa	5,6-dihydrouracil + NADP+	-	?

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Sus scrofa

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Release 2023.1 (January 2023)