Cloned (Comment) | Organism |
---|---|
overproduction of wild-type and mutant enzyme in Escherichia coli | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
C671A | 1% activity compared to that of the wild-type enzyme | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 16 iron atoms per enzyme, wild-type and mutant C671A, [4Fe-4S] clusters | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
111000 | - |
2 * 111000, SDS-PAGE | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thymine + NADPH | Sus scrofa | - |
dihydrothymine + NADP+ | - |
? | |
uracil + NADPH | Sus scrofa | rate limiting enzyme in pyrimidine degradation | 5,6-dihydrouracil + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzyme | Sus scrofa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5,6-dihydrouracil + NADP+ = uracil + NADPH + H+ | comparison of substrate and cofactor binding to wild-type and mutant enzyme | Sus scrofa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0043 | - |
mutant C671A | Sus scrofa |
0.43 | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6-dihydrouracil + NADP+ | C671A mutant only forms complex with dihydrouracil nearly without oxidizing it | Sus scrofa | uracil + NADPH | - |
r | |
thymine + NADPH | - |
Sus scrofa | dihydrothymine + NADP+ | - |
? | |
uracil + NADPH | - |
Sus scrofa | 5,6-dihydrouracil + NADP+ | - |
r | |
uracil + NADPH | rate limiting enzyme in pyrimidine degradation | Sus scrofa | 5,6-dihydrouracil + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 111000, SDS-PAGE | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | contains 2 mol FMN and 2 mol FAD per mol of enzyme, tightly associated | Sus scrofa | |
NADPH | - |
Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
NADP+ | - |
Sus scrofa |