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Literature summary for 1.3.1.2 extracted from

  • Shiotani, T.; Weber, G.
    Purification and properties of dihydrothymine dehydrogenase from rat liver (1981), J. Biol. Chem., 256, 219-224.
    View publication on PubMed

General Stability

General Stability Organism
2-mercaptoethanol stabilizes during dialysis and heating Rattus norvegicus
24 h dialysis against 35 mM potassium phosphate buffer, pH 7.4, with addition of 5 mM 2-mercaptoethanol or dithiothreitol, stable Rattus norvegicus
irreversible denaturation of enzyme in dilute solutions below 1 mg/ml Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
Uracil
-
Rattus norvegicus
0.0026
-
thymine
-
Rattus norvegicus
0.0029
-
NADP+ in presence of dihydrouracil Rattus norvegicus
0.0038
-
NADP+ in presence of dihydrothymine Rattus norvegicus
0.011
-
NADPH in presence of uracil Rattus norvegicus
0.015
-
NADPH in presence of thymine Rattus norvegicus
0.043
-
Dihydrothymine
-
Rattus norvegicus
0.193
-
dihydrouracil
-
Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 1 mol of enzyme contains 3 mol of iron Rattus norvegicus
Iron iron atoms are probably present in iron-sulfur centers Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
2 * 110000, SDS-PAGE Rattus norvegicus
220000
-
gel filtration, sedimentation equilibrium Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thymine + NADPH Rattus norvegicus rate-limiting enzyme of thymidine and uridine degradation dihydrothymine + NADP+
-
r
uracil + NADPH Rattus norvegicus
-
5,6-dihydrouracil + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.7
-
substrate nitouracil Rattus norvegicus
20.9
-
substrate 5-iodouracil Rattus norvegicus
21.5
-
substrate 5-diazouracil Rattus norvegicus
27.9
-
substrate thymine Rattus norvegicus
34.9
-
substrate uracil Rattus norvegicus
46.9
-
substrate 5-bromouracil Rattus norvegicus
47.4
-
substrate 5-fluorouracil Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,6-dihydrouracil + NADP+
-
Rattus norvegicus uracil + NADPH
-
r
5-bromouracil + NADPH
-
Rattus norvegicus 5-bromo-5,6-dihydrouracil + NADP+
-
?
5-diazouracil + NADPH
-
Rattus norvegicus ?
-
?
5-fluorouracil + NADPH + H+
-
Rattus norvegicus 5-fluoro-5,6-dihydrouracil + NADP+
-
?
5-iodouracil + NADPH
-
Rattus norvegicus 5-iodo-5,6-dihydrouracil + NADP+
-
?
5-nitrouracil + NADPH
-
Rattus norvegicus ?
-
?
dihydrothymine + NADP+
-
Rattus norvegicus thymine + NADPH
-
r
thymine + NADPH
-
Rattus norvegicus dihydrothymine + NADP+
-
r
thymine + NADPH rate-limiting enzyme of thymidine and uridine degradation Rattus norvegicus dihydrothymine + NADP+
-
r
uracil + NADPH
-
Rattus norvegicus 5,6-dihydrouracil + NADP+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 110000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
t1/2: 2 min without 2-mercaptoethanol, 2.5 min with 5 mM 2-mercaptoethanol Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
flavin enzyme contains 4 mol of FAD per mol of enzyme Rattus norvegicus
NADP+
-
Rattus norvegicus
NADPH
-
Rattus norvegicus