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Literature summary for 1.3.1.19 extracted from

  • Axcell, B.C.; Geary, P.J.
    The metabolism of benzene by bacteria. Purification and some properties of the enzyme cis-1,2-dihydroxycyclohexa-3,5-diene (nicotinamide adenine dinucleotide) oxidoreductase (cis-benzene glycol dehydrogenase) (1973), Biochem. J., 136, 927-934.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Fe2+
-
Pseudomonas sp.

General Stability

General Stability Organism
dilution, 0-4°C, sharp fall in activity in the first 5-10 h Pseudomonas sp.
loss of activity by ammonium sulfate precipitation and subsequent dialysis, restored by Fe2+ Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Pseudomonas sp.
p-chloromercuribenzoic acid
-
Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0435
-
NAD+
-
Pseudomonas sp.
0.286
-
cis-1,2-dihydrobenzene-1,2-diol cosubstrate NAD+ Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ optimum Fe2+ concentration 0.1 mM Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
4 * 110000, SDS-PAGE Pseudomonas sp.
440000
-
ultracentrifugation Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cis-1,2-dihydrobenzene-1,2-diol + NAD+ Pseudomonas sp.
-
catechol + NADH
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.1
-
cis-1,2-dihydrobenzene-1,2-diol, NAD+ Pseudomonas sp.

Storage Stability

Storage Stability Organism
0-4°C or frozen at -22°C, concentrated solution, several weeks Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-1,2-dihydrobenzene-1,2-diol + NAD+
-
Pseudomonas sp. catechol + NADH
-
?
cis-1,2-dihydrobenzene-1,2-diol + NAD+ specific for the cis-form of benzene glycol and for NAD+ as a hydrogen acceptor Pseudomonas sp. catechol + NADH
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 110000, SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
cis-1,2-dihydrocyclohexa-3,5-diene oxidoreductase
-
Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
enzyme assay Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
with NAD+ as cofactor and Fe2+ ions, 25 mM potassium phosphate buffer Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.9
-
in 25 mM-potassium phosphate buffer Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas sp.