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Literature summary for 1.3.1.14 extracted from

  • Friedmann, H.C.; Vennesland, B.
    Crystalline dihydroorotic dehydrogenase (1960), J. Biol. Chem., 235, 1526-1530.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cysteine essential for assay, interaction of enzyme with orotate or dihydroorotate is dependent on cysteine, whereas NADH oxidase activity is not Faecalicatena orotica
cysteine required for activation of purified enzyme, but not if crude extract is used for assay Faecalicatena orotica

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Faecalicatena orotica

Inhibitors

Inhibitors Comment Organism Structure
5-Methylorotate 2 mM, 50% inhibition of reduction of orotate Faecalicatena orotica
additional information not inhibited by 0.01 M arsenite Faecalicatena orotica
NaCl 0.2 M, 55% inhibition Faecalicatena orotica
p-chloromercuribenzoate
-
Faecalicatena orotica
phosphate high concentrations Faecalicatena orotica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
-
Faecalicatena orotica

Organism

Organism UniProt Comment Textmining
Faecalicatena orotica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Faecalicatena orotica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Faecalicatena orotica

Storage Stability

Storage Stability Organism
4°C, crystals, over a month, little or no loss of activity Faecalicatena orotica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-dihydroorotate + NAD+ substrate is oxidized by NAD+ and oxygen Faecalicatena orotica orotate + NADH + H+ lipoic acid is no substitute for orotate ?
5-fluorodihydroorotate + NAD+ more active substrate for reverse reaction than orotate Faecalicatena orotica 5-fluoroorotate + NADH + H+
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Faecalicatena orotica
25 30
-
Faecalicatena orotica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information values not per enzyme molecule, but per mol of flavin Faecalicatena orotica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
maximal activity Faecalicatena orotica

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein, ratio FAD to FMN 1:1 Faecalicatena orotica
FAD one molecule FAD and one molecule FMN per enzyme molecule Faecalicatena orotica
flavin flavoprotein with two different flavins, probably FAD and FMN Faecalicatena orotica
flavin 1 mol of flavin per 31 g of protein Faecalicatena orotica
FMN flavoprotein, ratio FAD to FMN 1:1 Faecalicatena orotica
FMN one molecule FAD and one molecule FMN per enzyme molecule Faecalicatena orotica
additional information one atom of iron bound per flavin Faecalicatena orotica