Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | essential for assay, interaction of enzyme with orotate or dihydroorotate is dependent on cysteine, whereas NADH oxidase activity is not | Faecalicatena orotica | |
cysteine | required for activation of purified enzyme, but not if crude extract is used for assay | Faecalicatena orotica |
Crystallization (Comment) | Organism |
---|---|
- |
Faecalicatena orotica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-Methylorotate | 2 mM, 50% inhibition of reduction of orotate | Faecalicatena orotica | |
additional information | not inhibited by 0.01 M arsenite | Faecalicatena orotica | |
NaCl | 0.2 M, 55% inhibition | Faecalicatena orotica | |
p-chloromercuribenzoate | - |
Faecalicatena orotica | |
phosphate | high concentrations | Faecalicatena orotica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
- |
Faecalicatena orotica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Faecalicatena orotica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Faecalicatena orotica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Faecalicatena orotica |
Storage Stability | Organism |
---|---|
4°C, crystals, over a month, little or no loss of activity | Faecalicatena orotica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + NAD+ | substrate is oxidized by NAD+ and oxygen | Faecalicatena orotica | orotate + NADH + H+ | lipoic acid is no substitute for orotate | ? | |
5-fluorodihydroorotate + NAD+ | more active substrate for reverse reaction than orotate | Faecalicatena orotica | 5-fluoroorotate + NADH + H+ | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Faecalicatena orotica |
25 | 30 | - |
Faecalicatena orotica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | values not per enzyme molecule, but per mol of flavin | Faecalicatena orotica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
maximal activity | Faecalicatena orotica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein, ratio FAD to FMN 1:1 | Faecalicatena orotica | |
FAD | one molecule FAD and one molecule FMN per enzyme molecule | Faecalicatena orotica | |
flavin | flavoprotein with two different flavins, probably FAD and FMN | Faecalicatena orotica | |
flavin | 1 mol of flavin per 31 g of protein | Faecalicatena orotica | |
FMN | flavoprotein, ratio FAD to FMN 1:1 | Faecalicatena orotica | |
FMN | one molecule FAD and one molecule FMN per enzyme molecule | Faecalicatena orotica | |
additional information | one atom of iron bound per flavin | Faecalicatena orotica |