Literature summary for 1.3.1.14 extracted from

  • Lieberman, I.; Kornberg, A.
    Enzymic synthesis and breakdown of a pyrimidine, orotic acid. I. Dihydro-orotic dehydrogenase (1953), Biochim. Biophys. Acta, 12, 223-234.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cysteine reaction rate increases 2fold in the presence of 2-7 mM cysteine Faecalicatena orotica

Inhibitors

Inhibitors Comment Organism Structure
cysteine inhibitory above 7 mM Faecalicatena orotica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
Orotate
-
Faecalicatena orotica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dihydroorotate + NAD+ Faecalicatena orotica
-
orotate + NADH + H+ breakdown of orotate r

Organism

Organism UniProt Comment Textmining
Faecalicatena orotica
-
obligate anaerobic soil bacterium
-

Purification (Commentary)

Purification (Comment) Organism
partial purification Faecalicatena orotica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Faecalicatena orotica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not: uracil, cytosine, 5-methylcytosine, thymine as substrates Faecalicatena orotica ?
-
?
(S)-dihydroorotate + NAD+ equilibrium favours direction of orotate reduction Faecalicatena orotica orotate + NADH + H+ orotate is identical with 4-carboxyuracil r
dihydroorotate + NAD+
-
Faecalicatena orotica orotate + NADH + H+ breakdown of orotate r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
pH of assay medium Faecalicatena orotica

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.8 pH 5.5: 13% of the activity at pH 6.5 Faecalicatena orotica
5.5 7.8 pH 7.8: 65% of the activity at pH 6.5 Faecalicatena orotica

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
less stable than at pH 6.5 Faecalicatena orotica
6.5
-
more stable than at pH 7.0 Faecalicatena orotica