BRENDA - Enzyme Database
show all sequences of 1.3.1.14

Purification and properties of diihydroorotic dehydrogenase

Friedmann, H.C.; Vennesland, B.; J. Biol. Chem. 233, 1398-1406 (1958)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
cysteine
essential for assay, interaction of enzyme with orotate or dihydroorotate is dependent on cysteine, whereas NADH oxidase activity is not
Faecalicatena orotica
glutathione
stimulating effect, but less effective than cysteine
Faecalicatena orotica
thioglycolate
stimulating effect, but less effective than cysteine
Faecalicatena orotica
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
1 mM, 10% inhibition
Faecalicatena orotica
2,4-Dihydroxy-6-methyl pyrimidine
-
Faecalicatena orotica
Acriflavin
partial inhibition
Faecalicatena orotica
Barbituric acid
-
Faecalicatena orotica
p-chloromercuribenzoate
completely inhibited by 0.1 mM
Faecalicatena orotica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.13
-
5-Fluoroorotate
-
Faecalicatena orotica
0.13
-
Orotate
-
Faecalicatena orotica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dihydroorotate + NAD+
Faecalicatena orotica
enzyme presumably functions mainly in the metabolic synthesis of pyrimidines
orotate + NADH + H+
-
Faecalicatena orotica
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Faecalicatena orotica
-
-
-
Purification (Commentary)
Commentary
Organism
about 90fold purification
Faecalicatena orotica
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Faecalicatena orotica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-dihydroorotate + NAD+
equilibrium favours direction of orotate reduction
390561
Faecalicatena orotica
orotate + NADH + H+
-
390561
Faecalicatena orotica
r
(S)-dihydroorotate + NAD+
substrate is oxidized by NAD+ and oxygen
390561
Faecalicatena orotica
orotate + NADH + H+
-
390561
Faecalicatena orotica
r
5-fluorodihydroorotate + NAD+
more active substrate for reverse reaction than orotate
390561
Faecalicatena orotica
5-fluoroorotate + NADH + H+
-
-
-
r
dihydroorotate + NAD+
enzyme presumably functions mainly in the metabolic synthesis of pyrimidines
390561
Faecalicatena orotica
orotate + NADH + H+
-
390561
Faecalicatena orotica
r
additional information
3-acetylpyridine-NAD+ is a more effective oxidant for dihydroorotate than NAD+, methylene blue can serve as oxidant, but not cytochrome c
390561
Faecalicatena orotica
?
-
-
-
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
values not per enzyme molecule, but per mol of flavin
Faecalicatena orotica
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
flavoprotein with two different flavins, probably FAD and FMN
Faecalicatena orotica
flavin
flavoprotein with two different flavins, probably FAD and FMN; interaction of flavin and dihydroorotate is completely dependent on cysteine
Faecalicatena orotica
FMN
flavoprotein with two different flavins, probably FAD and FMN
Faecalicatena orotica
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
cysteine
essential for assay, interaction of enzyme with orotate or dihydroorotate is dependent on cysteine, whereas NADH oxidase activity is not
Faecalicatena orotica
glutathione
stimulating effect, but less effective than cysteine
Faecalicatena orotica
thioglycolate
stimulating effect, but less effective than cysteine
Faecalicatena orotica
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
flavoprotein with two different flavins, probably FAD and FMN
Faecalicatena orotica
flavin
flavoprotein with two different flavins, probably FAD and FMN; interaction of flavin and dihydroorotate is completely dependent on cysteine
Faecalicatena orotica
FMN
flavoprotein with two different flavins, probably FAD and FMN
Faecalicatena orotica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
1 mM, 10% inhibition
Faecalicatena orotica
2,4-Dihydroxy-6-methyl pyrimidine
-
Faecalicatena orotica
Acriflavin
partial inhibition
Faecalicatena orotica
Barbituric acid
-
Faecalicatena orotica
p-chloromercuribenzoate
completely inhibited by 0.1 mM
Faecalicatena orotica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.13
-
5-Fluoroorotate
-
Faecalicatena orotica
0.13
-
Orotate
-
Faecalicatena orotica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dihydroorotate + NAD+
Faecalicatena orotica
enzyme presumably functions mainly in the metabolic synthesis of pyrimidines
orotate + NADH + H+
-
Faecalicatena orotica
r
Purification (Commentary) (protein specific)
Commentary
Organism
about 90fold purification
Faecalicatena orotica
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Faecalicatena orotica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-dihydroorotate + NAD+
equilibrium favours direction of orotate reduction
390561
Faecalicatena orotica
orotate + NADH + H+
-
390561
Faecalicatena orotica
r
(S)-dihydroorotate + NAD+
substrate is oxidized by NAD+ and oxygen
390561
Faecalicatena orotica
orotate + NADH + H+
-
390561
Faecalicatena orotica
r
5-fluorodihydroorotate + NAD+
more active substrate for reverse reaction than orotate
390561
Faecalicatena orotica
5-fluoroorotate + NADH + H+
-
-
-
r
dihydroorotate + NAD+
enzyme presumably functions mainly in the metabolic synthesis of pyrimidines
390561
Faecalicatena orotica
orotate + NADH + H+
-
390561
Faecalicatena orotica
r
additional information
3-acetylpyridine-NAD+ is a more effective oxidant for dihydroorotate than NAD+, methylene blue can serve as oxidant, but not cytochrome c
390561
Faecalicatena orotica
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
values not per enzyme molecule, but per mol of flavin
Faecalicatena orotica
Other publictions for EC 1.3.1.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
701809
Kawasaki
b-Type dihydroorotate dehydrog ...
Bifidobacterium bifidum
Appl. Environ. Microbiol.
75
629-636
2009
-
-
-
-
-
-
4
-
-
-
1
-
-
4
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
1
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
674586
Combe
Lys-D48 is required for charge ...
Lactococcus lactis
J. Biol. Chem.
281
17977-17988
2006
-
-
-
-
4
-
-
9
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
9
-
-
-
3
-
-
-
-
-
-
3
-
4
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
654759
Mohsen
Thermodynamic basis of electro ...
Lactococcus lactis
Biochemistry
43
6498-6510
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390568
Argyrou
Dihydroorotate dehydrogenase f ...
Bacillus subtilis, Enterococcus faecalis, Faecalicatena orotica, Lactococcus lactis
Biochemistry
39
10373-10384
2000
6
-
2
-
-
-
-
2
4
-
3
3
-
4
-
-
1
3
-
-
1
1
13
3
1
-
-
3
-
1
-
12
-
-
-
6
-
2
12
-
-
-
-
-
-
2
4
-
3
3
-
-
-
1
-
-
1
1
13
3
1
-
-
3
-
1
-
-
-
-
-
-
-
-
390569
Rowland
Structure of dihydroorotate de ...
Bacillus subtilis, Enterococcus faecalis, Faecalicatena orotica, Lactococcus lactis
Structure
8
1227-1238
2000
-
5
-
1
-
-
-
-
4
-
-
3
-
4
-
-
1
1
-
-
-
-
11
3
-
-
-
-
-
-
-
12
-
-
-
-
5
-
12
1
-
-
-
-
-
-
4
-
-
3
-
-
-
1
-
-
-
-
11
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390928
Kahler
Biochemical characterization o ...
Bacillus subtilis, Mus musculus
Arch. Biochem. Biophys.
371
191-201
1999
-
-
1
-
5
-
1
3
-
-
8
1
-
2
-
-
1
1
1
-
-
3
5
1
-
-
-
-
-
-
-
3
2
-
-
-
-
1
3
-
5
-
-
1
2
3
-
-
8
1
-
-
-
1
1
-
-
3
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
391244
Marcinkeviciene
Dihydroorotate dehydrogenase B ...
Enterococcus faecalis
Biochemistry
38
13129-13137
1999
-
-
-
-
-
-
-
-
-
-
3
-
-
3
-
-
1
1
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390917
Andersen
Two different dihydroorotate d ...
Lactococcus lactis
J. Bacteriol.
176
3975-3982
1994
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390565
Buntain
-
Latent Inhibitors. Part 4. Irr ...
Faecalicatena orotica
J. Chem. Soc. Perkin Trans. I
1988
3175-3182
1988
-
2
-
-
-
1
12
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
8
-
-
-
2
-
1
-
-
1
-
12
8
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
390564
Friedmann
-
Bestimmung mit Dihydroorotat-D ...
Faecalicatena orotica
Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )
2
2010-2014
1984
1
-
-
-
-
-
4
2
-
-
-
-
-
1
-
-
1
-
-
-
-
1
3
-
-
-
-
1
1
-
-
4
1
-
-
1
-
-
4
-
-
-
-
4
1
2
-
-
-
-
-
-
-
1
-
-
-
1
3
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
390563
Blattmann
Stereospecificity of the dihyd ...
Faecalicatena orotica
Eur. J. Biochem.
30
130-137
1972
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390567
Nelson
Preparation of bovine xanthine ...
Faecalicatena orotica
J. Biol. Chem.
243
5368-5373
1968
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
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-
-
2
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390566
Friedmann
Crystalline dihydroorotic dehy ...
Faecalicatena orotica
J. Biol. Chem.
235
1526-1530
1960
1
-
-
1
-
-
5
-
-
-
1
-
-
1
-
-
1
-
-
-
1
1
2
-
2
-
-
1
1
-
-
4
-
-
-
1
-
-
4
1
-
-
-
5
-
-
-
-
1
-
-
-
-
1
-
-
1
1
2
-
2
-
-
1
1
-
-
-
-
-
-
-
-
-
390561
Friedmann
Purification and properties of ...
Faecalicatena orotica
J. Biol. Chem.
233
1398-1406
1958
3
-
-
-
-
-
5
2
-
-
-
1
-
1
-
-
1
-
-
-
1
-
5
-
-
-
-
1
-
-
-
3
-
-
-
3
-
-
3
-
-
-
-
5
-
2
-
-
-
1
-
-
-
1
-
-
1
-
5
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
390562
Lieberman
Enzymic synthesis and breakdow ...
Faecalicatena orotica
Biochim. Biophys. Acta
12
223-234
1953
1
-
-
-
-
-
1
1
-
-
-
1
-
1
-
-
1
-
-
-
1
-
3
-
-
-
-
-
1
1
2
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
1
-
-
1
-
3
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-