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Literature summary for 1.3.1.13 extracted from

  • Holland, C.; Jez, J.
    Reaction mechanism of prephenate dehydrogenase from the alternative tyrosine biosynthesis pathway in plants (2018), ChemBioChem, 19, 1132-1136 .
    View publication on PubMed
Search for more literature for 1.3.1.13

Protein Variants

Protein Variants Comment Organism
H124A inactive Glycine max
H124N substantial decrease in catalytic efficiency Glycine max
H124Q inactive Glycine max
H188A inactive Glycine max
H188N inactive Glycine max
H188Q inactive Glycine max
N222A complete loss of activity Glycine max
N222Q 30fold decrease in kcat value and a modest change in Km value Glycine max
Q130A 300fold lower turnover rate Glycine max
Q130D 4fold reduction in kcat values and less than twofold increase in Km value Glycine max
Q130E 4fold reduction in kcat values and less than twofold increase in Km value Glycine max
Q130S 35fold lower turnover rate Glycine max
Q184A 320fold lower kcat value, with a Km value comparable to that of the wild-type Glycine max
Q184D loss of detectable PDH activity Glycine max
Q184E loss of detectable PDH activity Glycine max
S101A mutantion primarily affectes the Km value for prephenate with a 20fold increase Glycine max
T131A 134fold decrease in kcat value Glycine max
T131S 272fold decrease in kcat value Glycine max
T206A loss of detectable activity Glycine max
T206S tremendous loss of activity Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 prephenate mutant T206S, pH 7.5, 37°C Glycine max
0.17
-
 prephenate mutant T131S, pH 7.5, 37°C Glycine max
0.21
-
 prephenate mutant T131A, pH 7.5, 37°C Glycine max
0.22
-
 prephenate mutant Q130A, pH 7.5, 37°C Glycine max
0.26
-
 prephenate mutant Q130S, pH 7.5, 37°C Glycine max
0.27
-
 prephenate wild-type, pH 7.5, 37°C Glycine max
0.29
-
 prephenate mutant H124N, pH 7.5, 37°C Glycine max
0.37
-
 prephenate mutant Q184A, pH 7.5, 37°C Glycine max
0.44
-
 prephenate mutant Q130E, pH 7.5, 37°C Glycine max
0.48
-
 prephenate mutant Q130D, pH 7.5, 37°C Glycine max
0.49
-
 prephenate mutant N222Q, pH 7.5, 37°C Glycine max
5.2
-
 prephenate mutant S101A, pH 7.5, 37°C Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
prephenate + NADP+
-
 Glycine max 4-hydroxyphenylpyruvate + CO2 + NADPH
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.018
-
 prephenate mutant T206S, pH 7.5, 37°C Glycine max
0.052
-
 prephenate mutant Q184A, pH 7.5, 37°C Glycine max
0.055
-
 prephenate mutant Q130A, pH 7.5, 37°C Glycine max
0.06
-
 prephenate mutant T131S, pH 7.5, 37°C Glycine max
0.075
-
 prephenate mutant H124N, pH 7.5, 37°C Glycine max
0.122
-
 prephenate mutant T131A, pH 7.5, 37°C Glycine max
0.5
-
 prephenate mutant N222Q, pH 7.5, 37°C Glycine max
0.583
-
 prephenate mutant Q130S, pH 7.5, 37°C Glycine max
2.83
-
 prephenate mutant Q130D, pH 7.5, 37°C Glycine max
3.83
-
 prephenate mutant Q130E, pH 7.5, 37°C Glycine max
16.3
-
 prephenate wild-type, pH 7.5, 37°C Glycine max
18.3
-
 prephenate mutant S101A, pH 7.5, 37°C Glycine max

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.015
-
 prephenate mutant H124N, pH 7.5, 37°C Glycine max
0.13
-
 prephenate mutant T206S, pH 7.5, 37°C Glycine max
0.14
-
 prephenate mutant Q184A, pH 7.5, 37°C Glycine max
0.25
-
 prephenate mutant Q130A, pH 7.5, 37°C Glycine max
0.36
-
 prephenate mutant T131S, pH 7.5, 37°C Glycine max
0.58
-
 prephenate mutant T131A, pH 7.5, 37°C Glycine max
1.02
-
 prephenate mutant N222Q, pH 7.5, 37°C Glycine max
2.22
-
 prephenate mutant Q130S, pH 7.5, 37°C Glycine max
3.53
-
 prephenate mutant S101A, pH 7.5, 37°C Glycine max
5.9
-
 prephenate mutant Q130D, pH 7.5, 37°C Glycine max
8.71
-
 prephenate mutant Q130E, pH 7.5, 37°C Glycine max
60.5
-
 prephenate wild-type, pH 7.5, 37°C Glycine max