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Literature summary for 1.3.1.124 extracted from

  • Chu, X.; Yu, W.; Chen, G.; Li, D.
    Expression, purification, and characterization of His-tagged human mitochondrial 2,4-dienoyl-CoA reductase (2003), Protein Expr. Purif., 31, 292-297 .
    View publication on PubMed
Search for more literature for 1.3.1.124

Cloned(Commentary)

Cloned (Comment) Organism
expression of a highly active truncated soluble protein in Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0062
-
 (2E,4E)-2,4-decadienoyl-CoA pH 6.0, 23°C Homo sapiens
0.0265
-
 (2E,4E)-2,4-hexadienoyl-CoA pH 6.0, 23°C Homo sapiens
0.0605
-
 NADPH pH 6.0, 23°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q16698
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, one-step purification with a nickel HiTrap chelating metal affinity column Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.21
-
 pH 6.0, 23°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,4E)-2,4-decadienoyl-CoA + NADPH + H+ preferred substrate Homo sapiens (3E)-deca-3-enoyl-CoA + NADP+
-
 ?
(2E,4E)-2,4-hexadienoyl-CoA + NADPH + H+
-
 Homo sapiens (3E)-hexa-3-enoyl-CoA + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 34000, truncated recombinant protein, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
 Homo sapiens