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Literature summary for 1.3.1.124 extracted from

  • Mizugaki, M.; Koeda, N.; Kondo, A.; Kimura, C.; Yamanaka, H.
    Studies on the metabolism of unsaturated fatty acids. XVI. Purification and general properties of 2,4-dienoyl-CoA reductase from Candida lipolytica (1985), J. Biochem., 97, 837-843 .
    View publication on PubMed
Search for more literature for 1.3.1.124

Activating Compound

Activating Compound Comment Organism Structure
BSA 2-3fold incrase in activity in presence of BSA Yarrowia lipolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
360000
-
 gel filtration Yarrowia lipolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,4E)-2,4-decadienoyl-CoA + NADPH + H+ Yarrowia lipolytica
-
 (3E)-3-decaenoyl-CoA + NADP+
-
 ?
(2E,4E)-2,4-octadienoyl-CoA + NADPH + H+ Yarrowia lipolytica
-
 (3E)-3-octaenoyl-CoA + NADP+
-
 ?
(2E,4Z)-2,4-decadienoyl-CoA + NADPH + H+ Yarrowia lipolytica preferred over substrate (2E,4E)-2,4-decadienoyl-CoA (3E)-3-decaenoyl-CoA + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Yarrowia lipolytica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Yarrowia lipolytica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8
-
 substrate (2E,4E)-2,4-octadienoyl-CoA, pH 6.5, 25°C Yarrowia lipolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,4E)-2,4-decadienoyl-CoA + NADPH + H+
-
 Yarrowia lipolytica (3E)-3-decaenoyl-CoA + NADP+
-
 ?
(2E,4E)-2,4-octadienoyl-CoA + NADPH + H+
-
 Yarrowia lipolytica (3E)-3-octaenoyl-CoA + NADP+
-
 ?
(2E,4Z)-2,4-decadienoyl-CoA + NADPH + H+ preferred over substrate (2E,4E)-2,4-decadienoyl-CoA Yarrowia lipolytica (3E)-3-decaenoyl-CoA + NADP+
-
 ?

Subunits

Subunits Comment Organism
decamer or dodecamer, 12 * 33000 or 10 * 33000, SDS-PAGE Yarrowia lipolytica
dodecamer or decamer, 12 * 33000 or 10 * 33000, SDS-PAGE Yarrowia lipolytica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 10 min, 60-70% loss of activity Yarrowia lipolytica
50
-
 10 min, complete loss of activity Yarrowia lipolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Yarrowia lipolytica

Expression

Organism Comment Expression
Yarrowia lipolytica expression is induced by growth on linoleic acid up