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Literature summary for 1.3.1.124 extracted from

  • Fillgrove, K.L.; Anderson, V.E.; Mizugaki, M.
    Cloning, expression, and purification of the functional 2,4-dienoyl-CoA reductase from rat liver mitochondria (1999), Protein Expr. Purif., 17, 57-63.
    View publication on PubMed
Search for more literature for 1.3.1.124

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00046
-
 2-trans,4-trans-hexadienoyl-CoA
-
 Rattus norvegicus
0.0025
-
 NADPH
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32410
-
 purified recombinant enzyme, electrospray ionization-mass spectrometry Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ion-exchange and affinity chromatography Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1
-
 2-trans,4-trans-decadienoyl-CoA, NADPH Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-trans,4-trans-decadienoyl-CoA + NADPH
-
 Rattus norvegicus 3-decenoyl-CoA + NADP+
-
 ?
2-trans,4-trans-hexadienoyl-CoA + NADPH
-
 Rattus norvegicus ?
-
 ir

Synonyms

Synonyms Comment Organism
2,4-dienoyl-CoA reductase
-
 Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
 2-trans,4-trans-hexadienoyl-CoA
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Rattus norvegicus