Literature summary for 1.3.1.12 extracted from

Jiang, C.; Yin, B.; Tang, M.; Zhao, G.; He, J.; Shen, P.; Wu, B.
Identification of a metagenome-derived prephenate dehydrogenase gene from an alkaline-polluted soil microorganism (2013), Antonie van Leeuwenhoek, 103, 1209-1219.

Search for more literature for 1.3.1.12

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	uncultured bacterium

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	not inhibitory: tyrosine	uncultured bacterium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.87	-	prephenate	pH 8.0, 45°C	uncultured bacterium

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu2+	0.5 mM, 56.8% residual activity	uncultured bacterium
Fe3+	0.5 mM, 58.7% residual activity	uncultured bacterium
Mn2+	0.5 mM, 72% residual activity	uncultured bacterium
SDS	0.10%, 18.3% residual activity	uncultured bacterium

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31400	-	x * 31400, calculated	uncultured bacterium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
prephenate + NAD+	uncultured bacterium	-	4-hydroxyphenylpyruvate + NADH + H+ + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
uncultured bacterium	J9XQS6	uncultured alkaline-polluted bacterium	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	uncultured bacterium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
prephenate + NAD+	-	uncultured bacterium	4-hydroxyphenylpyruvate + NADH + H+ + CO2	-	?

Subunits

Subunits	Comment	Organism
?	x * 31400, calculated	uncultured bacterium

Synonyms

Synonyms	Comment	Organism
pdhE-1	-	uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	-	-	uncultured bacterium

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
35	50	more than 80% of maximum activity within	uncultured bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
10.08	-	prephenate	pH 8.0, 45°C	uncultured bacterium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	uncultured bacterium

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	8.5	more than 75% of maximum activity within	uncultured bacterium

pI Value

Organism	Comment	pI Value Maximum	pI Value
uncultured bacterium	-	-	5.5

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
11.6	-	prephenate	pH 8.0, 45°C	uncultured bacterium

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Release 2023.1 (January 2023)