Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Guanidine-HCl | activates at low concentrations, at higher concentrations concomitant loss of activity with a multi-state pathway of denaturation | Aquifex aeolicus |
Cloned (Comment) | Organism |
---|---|
into vector pET-15b, expression as His-tagged protein in Escherichia coli | Aquifex aeolicus |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-19 | delta19PD, more sensitive to temperature effects yielding a half-life of 55 min at 95°C versus 2 h for PD | Aquifex aeolicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | weakly inhibits | Aquifex aeolicus | |
additional information | EDTA, MgCl2 and ZnCl2 show no effect on enzyme activity | Aquifex aeolicus | |
tyrosine | feedback-inhibitor of activity over a wide temperature range, enhances the cooperativity between subunits in the binding of prephenate | Aquifex aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
prephenate | at 30°C, wild-type | Aquifex aeolicus | |
0.017 | - |
L-arogenate | at 30°C, wild-type | Aquifex aeolicus | |
0.025 | - |
NAD+ | at 30°C, wild-type | Aquifex aeolicus | |
0.041 | - |
prephenate | at 30°C, delta19PD | Aquifex aeolicus | |
0.041 | - |
L-arogenate | at 30°C, delta19PD | Aquifex aeolicus | |
0.053 | - |
NAD+ | at 30°C, delta19PD | Aquifex aeolicus | |
0.071 | - |
NAD+ | at 55°C, delta19PD | Aquifex aeolicus | |
0.072 | - |
NAD+ | at 55°C, wild-type | Aquifex aeolicus | |
0.084 | - |
NAD+ | at 80°C, delta19PD | Aquifex aeolicus | |
0.097 | - |
prephenate | at 55°C, wild-type | Aquifex aeolicus | |
0.097 | - |
L-arogenate | at 55°C, wild-type | Aquifex aeolicus | |
0.109 | - |
NAD+ | at 80°C, wild-type | Aquifex aeolicus | |
0.135 | - |
prephenate | at 55°C, delta19PD | Aquifex aeolicus | |
0.135 | - |
L-arogenate | at 55°C, delta19PD | Aquifex aeolicus | |
0.136 | - |
prephenate | at 80°C, wild-type | Aquifex aeolicus | |
0.136 | - |
L-arogenate | at 80°C, wild-type | Aquifex aeolicus | |
0.249 | - |
prephenate | at 80°C, delta19PD | Aquifex aeolicus | |
0.249 | - |
L-arogenate | at 80°C, delta19PD | Aquifex aeolicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | - |
Aquifex aeolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
1 * 37000 + 1 * 34000, SDS-PAGE | Aquifex aeolicus |
37000 | - |
1 * 37000 + 1 * 34000, SDS-PAGE | Aquifex aeolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by nickel-affinity chromatography | Aquifex aeolicus |
Storage Stability | Organism |
---|---|
-86°C | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arogenate + NAD+ | - |
Aquifex aeolicus | L-tyrosine + NADH + CO2 | - |
? | |
prephenate + NAD+ | - |
Aquifex aeolicus | 4-hydroxyphenylpyruvate + CO2 + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 37000 + 1 * 34000, SDS-PAGE | Aquifex aeolicus |
Synonyms | Comment | Organism |
---|---|---|
PD | - |
Aquifex aeolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Aquifex aeolicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 95 | - |
Aquifex aeolicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
NAD+ | at 30°C, wild-type | Aquifex aeolicus | |
1.3 | - |
prephenate | at 30°C, wild-type | Aquifex aeolicus | |
1.3 | - |
L-arogenate | at 30°C, wild-type | Aquifex aeolicus | |
1.5 | - |
NAD+ | at 30°C, delta19PD | Aquifex aeolicus | |
2.2 | - |
prephenate | at 30°C, delta19PD | Aquifex aeolicus | |
2.2 | - |
L-arogenate | at 30°C, delta19PD | Aquifex aeolicus | |
3 | 6 | prephenate | at 80°C, wild-type | Aquifex aeolicus | |
3 | 6 | L-arogenate | at 80°C, wild-type | Aquifex aeolicus | |
6.7 | - |
NAD+ | at 55°C, wild-type | Aquifex aeolicus | |
6.8 | - |
prephenate | at 55°C, wild-type | Aquifex aeolicus | |
6.8 | - |
L-arogenate | at 55°C, wild-type | Aquifex aeolicus | |
11.5 | - |
NAD+ | at 55°C, delta19PD | Aquifex aeolicus | |
13 | - |
prephenate | at 55°C, delta19PD | Aquifex aeolicus | |
13 | - |
L-arogenate | at 55°C, delta19PD | Aquifex aeolicus | |
34 | - |
NAD+ | at 80°C, wild-type | Aquifex aeolicus | |
62 | - |
NAD+ | at 80°C, delta19PD | Aquifex aeolicus | |
68 | - |
prephenate | at 80°C, delta19PD | Aquifex aeolicus | |
68 | - |
L-arogenate | at 80°C, delta19PD | Aquifex aeolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
at 55°C | Aquifex aeolicus |