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Literature summary for 1.3.1.119 extracted from
- Purification and characterization of chlorobenzene cis-dihydrodiol dehydrogenase from Xanthobacter flavus 14p1 (1996), Arch. Microbiol., 165, 201-205 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 8-hydroxyquinoline | 1 mM, 54% inhibition | Xanthobacter flavus |  |
| HgCl2 | 0.1 mM, complete loss of activity, most potent inhibitor | Xanthobacter flavus | |
| KCN | 1 mM, 26% inhibition | Xanthobacter flavus | |
| additional information | no inhibition by EDTA, 2,2-dipyridyl, and 1,10-phenanthroline | Xanthobacter flavus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
NAD+ | pH 8.0, 30°C, cosubstrate: 3,6-dichlorobenzene cis-dihydrodiol | Xanthobacter flavus | |
| 0.02 | - |
NAD+ | pH 8.0, 30°C, cosubstrate: benzene cis-dihydrodiol | Xanthobacter flavus | |
| 0.21 | - |
3,6-dichlorobenzene cis-dihydrodiol | pH 8.0, 30°C | Xanthobacter flavus | |
| 0.78 | - |
benzene cis-dihydrodiol | pH 8.0, 30°C | Xanthobacter flavus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
gel filtration | Xanthobacter flavus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Xanthobacter flavus | - |
- |
- |
| Xanthobacter flavus DSM 10330 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Xanthobacter flavus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 32 | - |
substrate: 3,6-dichlorobenzene cis-dihydrodiol, pH 8.0, 30°C | Xanthobacter flavus |
| 63 | - |
substrate: benzene cis-dihydrodiol, pH 8.0, 30°C | Xanthobacter flavus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dichlorobenzene cis-dihydrodiol + NAD+ | activity is 30% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus | 3,4-dichlorocatechol + NADH + H+ | - |
? | |
| 3,4-dichlorobenzene cis-dihydrodiol + NAD+ | activity is 30% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus DSM 10330 | 3,4-dichlorocatechol + NADH + H+ | - |
? | |
| 3,5-dichlorobenzene cis-dihydrodiol + NAD+ | activity is 195% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus | 3,5-dichlorocatechol + NADH + H+ | - |
? | |
| 3,5-dichlorobenzene cis-dihydrodiol + NAD+ | activity is 195% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus DSM 10330 | 3,5-dichlorocatechol + NADH + H+ | - |
? | |
| 3,6-dichlorobenzene cis-dihydrodiol + NAD+ | activity is 60% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus | 3,6-dichlorocatechol + NADH + H+ | - |
? | |
| 3,6-dichlorobenzene cis-dihydrodiol + NAD+ | activity is 60% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus DSM 10330 | 3,6-dichlorocatechol + NADH + H+ | - |
? | |
| 3,6-dimethylbenzene cis-dihydrodiol + NAD+ | activity is 105% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus | 3,6-dimethylcatechol + NADH + H+ | - |
? | |
| 3,6-dimethylbenzene cis-dihydrodiol + NAD+ | activity is 105% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus DSM 10330 | 3,6-dimethylcatechol + NADH + H+ | - |
? | |
| 3-chlorobenzene cis-dihydrodiol + NAD+ | activity is 170% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus | 3-chlorocatechol + NADH + H+ | - |
? | |
| 3-chlorobenzene cis-dihydrodiol + NAD+ | activity is 170% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus DSM 10330 | 3-chlorocatechol + NADH + H+ | - |
? | |
| 3-fluorobenzene cis-dihydrodiol + NAD+ | activity is 145% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus | 3-fluorocatechol + NADH + H+ | - |
? | |
| 3-methylbenzene cis-dihydrodiol + NAD+ | activity is 185% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus | 3-methylcatechol + NADH + H+ | - |
? | |
| benzene cis-dihydrodiol + NAD+ | - |
Xanthobacter flavus | catechol + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 26500, SDS-PAGE | Xanthobacter flavus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3,6-dichlorobenzene DDH | - |
Xanthobacter flavus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14.5 | - |
3,6-dichlorobenzene cis-dihydrodiol | pH 8.0, 30°C | Xanthobacter flavus | |
| 14.5 | - |
NAD+ | pH 8.0, 30°C, cosubstrate: 3,6-dichlorobenzene cis-dihydrodiol | Xanthobacter flavus | |
| 27.8 | - |
benzene cis-dihydrodiol | pH 8.0, 30°C | Xanthobacter flavus | |
| 27.8 | - |
NAD+ | pH 8.0, 30°C, cosubstrate: benzene cis-dihydrodiol | Xanthobacter flavus | |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
optimum stability at 4°C | Xanthobacter flavus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | the enzyme accepts also NADP+ as cosubstrate. The activity with NADP+ is reduced to 14% of that with NAD+ | Xanthobacter flavus | |
| NADP+ | the activity with NADP+ is reduced to 14% of that with NAD+ | Xanthobacter flavus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Xanthobacter flavus | - |
- |
5.4 |
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