Identification and cloning of an NADPH-dependent hydroxycinnamoyl-CoA double bond reductase involved in dihydrochalcone formation in Malus x domestica Borkh

Ibdah, M.; Berim, A.; Martens, S.; Valderrama, A.L.; Palmieri, L.; Lewinsohn, E.; Gang, D.R.; Phytochemistry 107, 24-31 (2014)

Data extracted from this reference:

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.093

-

trans-feruloyl-CoA

pH 50, 35°C

Malus domestica

0.097

-

trans-4-coumaroyl-CoA

pH 50, 35°C

Malus domestica

0.101

-

NADPH

pH 50, 35°C

Malus domestica

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

trans-4-coumaroyl-CoA + NADPH + H+

Malus domestica

enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1

dihydro-4-coumaroyl-CoA + NADP+

-

-

?

trans-feruloyl-CoA + NADPH + H+

Malus domestica

enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1

dihydroferuloyl-CoA + NADP+

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

trans-4-coumaroyl-CoA + NADPH + H+

-

745999

Malus domestica

dihydro-4-coumaroyl-CoA + NADP+

-

-

-

?

trans-4-coumaroyl-CoA + NADPH + H+

enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1

745999

Malus domestica

dihydro-4-coumaroyl-CoA + NADP+

-

-

-

?

trans-feruloyl-CoA + NADPH + H+

-

745999

Malus domestica

dihydroferuloyl-CoA + NADP+

-

-

-

?

trans-feruloyl-CoA + NADPH + H+

enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1

745999

Malus domestica

dihydroferuloyl-CoA + NADP+

-

-

-

?

Turnover Number Minimum [1/s]

Turnover Number Maximum [1/s]

Substrate

Commentary

Organism

Structure

0.0019

-

trans-4-coumaroyl-CoA

pH 50, 35°C

Malus domestica

0.0023

-

NADPH

pH 50, 35°C

Malus domestica

0.0042

-

trans-feruloyl-CoA

pH 50, 35°C

Malus domestica

Cofactor

Commentary

Organism

Structure

NADP+

-

Malus domestica

NADPH

-

Malus domestica

Cofactor

Commentary

Organism

Structure

NADP+

-

Malus domestica

NADPH

-

Malus domestica

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.093

-

trans-feruloyl-CoA

pH 50, 35°C

Malus domestica

0.097

-

trans-4-coumaroyl-CoA

pH 50, 35°C

Malus domestica

0.101

-

NADPH

pH 50, 35°C

Malus domestica

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

trans-4-coumaroyl-CoA + NADPH + H+

Malus domestica

enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1

dihydro-4-coumaroyl-CoA + NADP+

-

-

?

trans-feruloyl-CoA + NADPH + H+

Malus domestica

enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1

dihydroferuloyl-CoA + NADP+

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

trans-4-coumaroyl-CoA + NADPH + H+

-

745999

Malus domestica

dihydro-4-coumaroyl-CoA + NADP+

-

-

-

?

trans-4-coumaroyl-CoA + NADPH + H+

enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1

745999

Malus domestica

dihydro-4-coumaroyl-CoA + NADP+

-

-

-

?

trans-feruloyl-CoA + NADPH + H+

-

745999

Malus domestica

dihydroferuloyl-CoA + NADP+

-

-

-

?

trans-feruloyl-CoA + NADPH + H+

enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1

745999

Malus domestica

dihydroferuloyl-CoA + NADP+

-

-

-

?

Turnover Number Minimum [1/s]

Turnover Number Maximum [1/s]

Substrate

Commentary

Organism

Structure

0.0019

-

trans-4-coumaroyl-CoA

pH 50, 35°C

Malus domestica

0.0023

-

NADPH

pH 50, 35°C

Malus domestica

0.0042

-

trans-feruloyl-CoA

pH 50, 35°C

Malus domestica

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

745999

Ibdah

Identification and cloning of ...

Malus domestica

Phytochemistry

107

24-31

2014

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