BRENDA - Enzyme Database
show all sequences of 1.3.1.117

Identification and cloning of an NADPH-dependent hydroxycinnamoyl-CoA double bond reductase involved in dihydrochalcone formation in Malus x domestica Borkh

Ibdah, M.; Berim, A.; Martens, S.; Valderrama, A.L.; Palmieri, L.; Lewinsohn, E.; Gang, D.R.; Phytochemistry 107, 24-31 (2014)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Malus domestica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.093
-
trans-feruloyl-CoA
pH 50, 35°C
Malus domestica
0.097
-
trans-4-coumaroyl-CoA
pH 50, 35°C
Malus domestica
0.101
-
NADPH
pH 50, 35°C
Malus domestica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
trans-4-coumaroyl-CoA + NADPH + H+
Malus domestica
enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1
dihydro-4-coumaroyl-CoA + NADP+
-
-
?
trans-feruloyl-CoA + NADPH + H+
Malus domestica
enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1
dihydroferuloyl-CoA + NADP+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Malus domestica
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
trans-4-coumaroyl-CoA + NADPH + H+
-
745999
Malus domestica
dihydro-4-coumaroyl-CoA + NADP+
-
-
-
?
trans-4-coumaroyl-CoA + NADPH + H+
enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1
745999
Malus domestica
dihydro-4-coumaroyl-CoA + NADP+
-
-
-
?
trans-feruloyl-CoA + NADPH + H+
-
745999
Malus domestica
dihydroferuloyl-CoA + NADP+
-
-
-
?
trans-feruloyl-CoA + NADPH + H+
enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1
745999
Malus domestica
dihydroferuloyl-CoA + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 38400, SDS-PAGE and calculated from sequence
Malus domestica
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Malus domestica
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0019
-
trans-4-coumaroyl-CoA
pH 50, 35°C
Malus domestica
0.0023
-
NADPH
pH 50, 35°C
Malus domestica
0.0042
-
trans-feruloyl-CoA
pH 50, 35°C
Malus domestica
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Malus domestica
NADPH
-
Malus domestica
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Malus domestica
calculated from sequence
-
6.9
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Malus domestica
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Malus domestica
NADPH
-
Malus domestica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.093
-
trans-feruloyl-CoA
pH 50, 35°C
Malus domestica
0.097
-
trans-4-coumaroyl-CoA
pH 50, 35°C
Malus domestica
0.101
-
NADPH
pH 50, 35°C
Malus domestica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
trans-4-coumaroyl-CoA + NADPH + H+
Malus domestica
enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1
dihydro-4-coumaroyl-CoA + NADP+
-
-
?
trans-feruloyl-CoA + NADPH + H+
Malus domestica
enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1
dihydroferuloyl-CoA + NADP+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
trans-4-coumaroyl-CoA + NADPH + H+
-
745999
Malus domestica
dihydro-4-coumaroyl-CoA + NADP+
-
-
-
?
trans-4-coumaroyl-CoA + NADPH + H+
enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1
745999
Malus domestica
dihydro-4-coumaroyl-CoA + NADP+
-
-
-
?
trans-feruloyl-CoA + NADPH + H+
-
745999
Malus domestica
dihydroferuloyl-CoA + NADP+
-
-
-
?
trans-feruloyl-CoA + NADPH + H+
enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1
745999
Malus domestica
dihydroferuloyl-CoA + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 38400, SDS-PAGE and calculated from sequence
Malus domestica
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Malus domestica
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0019
-
trans-4-coumaroyl-CoA
pH 50, 35°C
Malus domestica
0.0023
-
NADPH
pH 50, 35°C
Malus domestica
0.0042
-
trans-feruloyl-CoA
pH 50, 35°C
Malus domestica
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Malus domestica
calculated from sequence
-
6.9
Other publictions for EC 1.3.1.117
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745999
Ibdah
Identification and cloning of ...
Malus domestica
Phytochemistry
107
24-31
2014
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3
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4
1
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1
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3
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2
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1
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1
2
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3
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2
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4
1
1
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3
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1
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