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Literature summary for 1.3.1.116 extracted from

  • Kang, D.; Ridlon, J.; Moore II, D.; Barnes, S.; Hylemon, P.
    Clostridium scindens baiCD and baiH genes encode stereo-specific 7alpha/7beta-hydroxy-3-oxo-DELTA4-cholenoic acid oxidoreductases (2008), Biochim. Biophys. Acta, 1781, 16-25 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli [Clostridium] scindens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
SDS-PAGE [Clostridium] scindens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens
-
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens the enzyme participates in the bile acid 7beta-dehydroxylation pathway 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens JCM 10418
-
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens JCM 10418 the enzyme participates in the bile acid 7beta-dehydroxylation pathway 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens VPI 12708
-
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ [Clostridium] scindens VPI 12708 the enzyme participates in the bile acid 7beta-dehydroxylation pathway 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
[Clostridium] scindens P32370
-
-
[Clostridium] scindens JCM 10418 P32370
-
-
[Clostridium] scindens VPI 12708 P32370
-
-

Purification (Commentary)

Purification (Comment) Organism
-
[Clostridium] scindens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.4
-
37°C, pH 6.8 [Clostridium] scindens
16.4
-
pH 6.8, 25°C [Clostridium] scindens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
-
[Clostridium] scindens 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7beta-dehydroxylation pathway [Clostridium] scindens 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme catalyses the stereo-specific oxidation of its substrate and has no activity with the 7alpha anomer [Clostridium] scindens 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
-
[Clostridium] scindens JCM 10418 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7beta-dehydroxylation pathway [Clostridium] scindens JCM 10418 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme catalyses the stereo-specific oxidation of its substrate and has no activity with the 7alpha anomer [Clostridium] scindens JCM 10418 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+
-
[Clostridium] scindens VPI 12708 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme participates in the bile acid 7beta-dehydroxylation pathway [Clostridium] scindens VPI 12708 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ the enzyme catalyses the stereo-specific oxidation of its substrate and has no activity with the 7alpha anomer [Clostridium] scindens VPI 12708 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+
-
?

Subunits

Subunits Comment Organism
? x * 72000, SDS-PAGE [Clostridium] scindens
homotrimer 3 * 72000, SDS-PAGE [Clostridium] scindens

Synonyms

Synonyms Comment Organism
7beta-hydroxy-3-oxo-DELTA4-cholenoic acid oxidoreductase
-
[Clostridium] scindens
BaiH
-
[Clostridium] scindens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at [Clostridium] scindens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at [Clostridium] scindens

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein containing 1 mol of FMN and 1 mol of FAD per subunit [Clostridium] scindens
FMN flavoprotein containing 1 mol of FMN and 1 mol of FAD per subunit [Clostridium] scindens
additional information no cofactor: NADP+ [Clostridium] scindens
NAD+
-
[Clostridium] scindens
NADH
-
[Clostridium] scindens

General Information

General Information Comment Organism
metabolism the enzyme participates in the bile acid 7beta-dehydroxylation pathway [Clostridium] scindens