Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | [Clostridium] scindens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
SDS-PAGE | [Clostridium] scindens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ | [Clostridium] scindens | - |
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+ | - |
? | |
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ | [Clostridium] scindens | the enzyme participates in the bile acid 7beta-dehydroxylation pathway | 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+ | - |
? | |
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ | [Clostridium] scindens JCM 10418 | - |
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+ | - |
? | |
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ | [Clostridium] scindens JCM 10418 | the enzyme participates in the bile acid 7beta-dehydroxylation pathway | 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+ | - |
? | |
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ | [Clostridium] scindens VPI 12708 | - |
7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+ | - |
? | |
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ | [Clostridium] scindens VPI 12708 | the enzyme participates in the bile acid 7beta-dehydroxylation pathway | 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Clostridium] scindens | P32370 | - |
- |
[Clostridium] scindens JCM 10418 | P32370 | - |
- |
[Clostridium] scindens VPI 12708 | P32370 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
[Clostridium] scindens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.4 | - |
37°C, pH 6.8 | [Clostridium] scindens |
16.4 | - |
pH 6.8, 25°C | [Clostridium] scindens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ | - |
[Clostridium] scindens | 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+ | - |
? | |
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ | the enzyme participates in the bile acid 7beta-dehydroxylation pathway | [Clostridium] scindens | 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+ | - |
? | |
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ | the enzyme catalyses the stereo-specific oxidation of its substrate and has no activity with the 7alpha anomer | [Clostridium] scindens | 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+ | - |
? | |
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ | - |
[Clostridium] scindens JCM 10418 | 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+ | - |
? | |
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ | the enzyme participates in the bile acid 7beta-dehydroxylation pathway | [Clostridium] scindens JCM 10418 | 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+ | - |
? | |
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ | the enzyme catalyses the stereo-specific oxidation of its substrate and has no activity with the 7alpha anomer | [Clostridium] scindens JCM 10418 | 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+ | - |
? | |
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ | - |
[Clostridium] scindens VPI 12708 | 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+ | - |
? | |
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ | the enzyme participates in the bile acid 7beta-dehydroxylation pathway | [Clostridium] scindens VPI 12708 | 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+ | - |
? | |
7beta-hydroxy-3-oxochol-24-oyl-CoA + NAD+ | the enzyme catalyses the stereo-specific oxidation of its substrate and has no activity with the 7alpha anomer | [Clostridium] scindens VPI 12708 | 7beta-hydroxy-3-oxochol-4-en-24-oyl-CoA + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 72000, SDS-PAGE | [Clostridium] scindens |
homotrimer | 3 * 72000, SDS-PAGE | [Clostridium] scindens |
Synonyms | Comment | Organism |
---|---|---|
7beta-hydroxy-3-oxo-DELTA4-cholenoic acid oxidoreductase | - |
[Clostridium] scindens |
BaiH | - |
[Clostridium] scindens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | [Clostridium] scindens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | [Clostridium] scindens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein containing 1 mol of FMN and 1 mol of FAD per subunit | [Clostridium] scindens | |
FMN | flavoprotein containing 1 mol of FMN and 1 mol of FAD per subunit | [Clostridium] scindens | |
additional information | no cofactor: NADP+ | [Clostridium] scindens | |
NAD+ | - |
[Clostridium] scindens | |
NADH | - |
[Clostridium] scindens |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme participates in the bile acid 7beta-dehydroxylation pathway | [Clostridium] scindens |