Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vitis vinifera | Q5FB34 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 cyanidin + 4 NADPH + 4 H+ | - |
Vitis vinifera | (+)-epicatechin + (-)-catechin + 4 NADP+ | at pH 7.5 and 30°C, the first hydride transfer to anthocyanidin is irreversible, and no intermediate is released during catalysis | ir | |
2 delphinidin + 4 NADPH + 4 H+ | - |
Vitis vinifera | (-)-gallocatechin + (+)-epigallocatechin + 4 NADP+ | - |
ir | |
2 pelargonidin + 4 NADPH + 4 H+ | - |
Vitis vinifera | (-)-afzelechin + (+)-epiafzelechin + 4 NADP+ | - |
ir | |
additional information | in the presence of excess NADP+, ANR acts as a flavan-3-ol C3-epimerase, but only with 2R-flavan-3-ols, not with 2S-flavan-3-ols produced by the enzyme in the forward reaction. C3-epimerization should be achieved by tautomerization between the two hydride transfers and this produces a quinone methide intermediate which serves as C4 target of the second hydride transfer, avoiding any stereospecific modification of carbon 3. The inversion of C2 stereochemistry required for reverse epimerization suggests that the 2S configuration induces an irreversible product dissociation | Vitis vinifera | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ANR | - |
Vitis vinifera |