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Literature summary for 1.3.1.110 extracted from

  • Weghoff, M.C.; Bertsch, J.; Müller, V.
    A novel mode of lactate metabolism in strictly anaerobic bacteria (2015), Environ. Microbiol., 17, 670-677.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 divalent cations stimulate activity, highest stimulation observed with CaCl2 is twofold. Activity increased linearly with increasing CaCl2 concentration and reaches saturation at 40 mM Acetobacterium woodii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
1 * 51000 (D-lactate dehydrogenase glycolate oxidase subunit GlcD) + 1 * 46000 (electron transfer flavoprotein alpha subunit) + 1 * 29000 (electron transfer flavoprotein beta), SDS-PAGE Acetobacterium woodii
46000
-
1 * 51000 (D-lactate dehydrogenase glycolate oxidase subunit GlcD) + 1 * 46000 (electron transfer flavoprotein alpha subunit) + 1 * 29000 (electron transfer flavoprotein beta), SDS-PAGE Acetobacterium woodii
51000
-
1 * 51000 (D-lactate dehydrogenase glycolate oxidase subunit GlcD) + 1 * 46000 (electron transfer flavoprotein alpha subunit) + 1 * 29000 (electron transfer flavoprotein beta), SDS-PAGE Acetobacterium woodii
138000
-
gel filtration Acetobacterium woodii
151000
-
native gel electrophoresis Acetobacterium woodii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster Acetobacterium woodii lactate is a common substrate for major groups of strictly anaerobic bacteria. The LDH/Etf complex of Acetobacterium woodii uses flavin-based electron confurcation to drive endergonic lactate oxidation with NAD+ as oxidant at the expense of simultaneous exergonic electron flow from reduced ferredoxin to NAD+ pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster
-
r
lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster Acetobacterium woodii DSM 1030 lactate is a common substrate for major groups of strictly anaerobic bacteria. The LDH/Etf complex of Acetobacterium woodii uses flavin-based electron confurcation to drive endergonic lactate oxidation with NAD+ as oxidant at the expense of simultaneous exergonic electron flow from reduced ferredoxin to NAD+ pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster
-
r

Organism

Organism UniProt Comment Textmining
Acetobacterium woodii H6LBB0 and H6LBB1 and H6LBS1 H6LBB0: electron transfer flavoprotein beta subunit, H6LBB1: electron transfer flavoprotein alpha subunit, H6LBS1: D-lactate dehydrogenase subunit GlcD
-
Acetobacterium woodii DSM 1030 H6LBB0 and H6LBB1 and H6LBS1 H6LBB0: electron transfer flavoprotein beta subunit, H6LBB1: electron transfer flavoprotein alpha subunit, H6LBS1: D-lactate dehydrogenase subunit GlcD
-

Purification (Commentary)

Purification (Comment) Organism
-
Acetobacterium woodii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster lactate is a common substrate for major groups of strictly anaerobic bacteria. The LDH/Etf complex of Acetobacterium woodii uses flavin-based electron confurcation to drive endergonic lactate oxidation with NAD+ as oxidant at the expense of simultaneous exergonic electron flow from reduced ferredoxin to NAD+ Acetobacterium woodii pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster
-
r
lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster pyruvate reduction to lactate with NADH as electron donor is highly exergonic Acetobacterium woodii pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster
-
r
lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster lactate is a common substrate for major groups of strictly anaerobic bacteria. The LDH/Etf complex of Acetobacterium woodii uses flavin-based electron confurcation to drive endergonic lactate oxidation with NAD+ as oxidant at the expense of simultaneous exergonic electron flow from reduced ferredoxin to NAD+ Acetobacterium woodii DSM 1030 pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster
-
r
lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster pyruvate reduction to lactate with NADH as electron donor is highly exergonic Acetobacterium woodii DSM 1030 pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster
-
r
pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster no NADH oxidation with pyruvate as electron acceptor. Only when oxidized ferredoxin is added to the system, the reaction is initiated and NADH oxidation is detected by a decrease of absorbance. NADH-dependent ferredoxin reduction is strictly dependent on the presence of lactate Acetobacterium woodii lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster
-
r
pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster no NADH oxidation with pyruvate as electron acceptor. Only when oxidized ferredoxin is added to the system, the reaction is initiated and NADH oxidation is detected by a decrease of absorbance. NADH-dependent ferredoxin reduction is strictly dependent on the presence of lactate Acetobacterium woodii DSM 1030 lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster
-
r

Subunits

Subunits Comment Organism
heterotrimer 1 * 51000 (D-lactate dehydrogenase glycolate oxidase subunit GlcD) + 1 * 46000 (electron transfer flavoprotein alpha subunit) + 1 * 29000 (electron transfer flavoprotein beta), SDS-PAGE Acetobacterium woodii

Synonyms

Synonyms Comment Organism
LDH/Etf complex
-
Acetobacterium woodii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 30
-
Acetobacterium woodii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 50 10°C: 65% of maximal activity, activity is optimal between 20 and 30°C. Activity above 30°C decreases by 20% and dropps down to only residual activity at 50°C Acetobacterium woodii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Acetobacterium woodii

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 activity is rather insensitive to a pH ranging from 5.5 to 9.0 Acetobacterium woodii

Cofactor

Cofactor Comment Organism Structure
FAD the LDH/Etf complex has three predicted FADs. FAD is required for stability and activity. FAD (0.005 mM) is present during all purification steps, as the enzyme precipitates in the absence of FAD. FMN can not substitute FAD Acetobacterium woodii
[4Fe-4S]-center the LDH/Etf complex has one predicted 4Fe-4S cluster Acetobacterium woodii

General Information

General Information Comment Organism
metabolism lactate is a common substrate for major groups of strictly anaerobic bacteria. The LDH/Etf complex of Acetobacterium woodii uses flavin-based electron confurcation to drive endergonic lactate oxidation with NAD+ as oxidant at the expense of simultaneous exergonic electron flow from reduced ferredoxin to NAD+ Acetobacterium woodii