BRENDA - Enzyme Database
show all sequences of 1.3.1.11

The metabolism of coumarin by a microorganism. II. The reduction of o-coumaric acid to melilotic acid

Levy, C.C.; Weinstein, G.D.; Biochemistry 3, 1944-1947 (1964) View publication on PubMed

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
iodoacetate
3.3 mM, 29% inhibition
Arthrobacter sp.
N-ethylmaleimide
3.0 mM, 63% inhibition
Arthrobacter sp.
p-chloromercuribenzoate
3.0 mM, 100% inhibition, 0.33 mM, 37% inhibition
Arthrobacter sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0173
-
o-coumarate
-
Arthrobacter sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-coumarate + NADH
Arthrobacter sp.
-
melilotate + NAD+
-
Arthrobacter sp.
?
Organism
Organism
UniProt
Commentary
Textmining
Arthrobacter sp.
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
partially
Arthrobacter sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.56
-
-
Arthrobacter sp.
Storage Stability
Storage Stability
Organism
1 week, -20°C
Arthrobacter sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2,4-dihydroxycinnamic acid + NADH
1.6% of activity with 2-coumarate
390510
Arthrobacter sp.
?
-
-
-
?
2-coumarate + NADH
-
390510
Arthrobacter sp.
melilotate + NAD+
-
390510
Arthrobacter sp.
?
2-coumarate + NADH
-
390510
Arthrobacter sp.
melilotate + NAD+
-
390510
Arthrobacter sp.
ir
4-coumarate + NADH
1.4% of activity with 2-coumarate
390510
Arthrobacter sp.
?
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
8.5
o-coumarate + NADH
Arthrobacter sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Arthrobacter sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Arthrobacter sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
iodoacetate
3.3 mM, 29% inhibition
Arthrobacter sp.
N-ethylmaleimide
3.0 mM, 63% inhibition
Arthrobacter sp.
p-chloromercuribenzoate
3.0 mM, 100% inhibition, 0.33 mM, 37% inhibition
Arthrobacter sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0173
-
o-coumarate
-
Arthrobacter sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-coumarate + NADH
Arthrobacter sp.
-
melilotate + NAD+
-
Arthrobacter sp.
?
Purification (Commentary) (protein specific)
Commentary
Organism
partially
Arthrobacter sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.56
-
-
Arthrobacter sp.
Storage Stability (protein specific)
Storage Stability
Organism
1 week, -20°C
Arthrobacter sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2,4-dihydroxycinnamic acid + NADH
1.6% of activity with 2-coumarate
390510
Arthrobacter sp.
?
-
-
-
?
2-coumarate + NADH
-
390510
Arthrobacter sp.
melilotate + NAD+
-
390510
Arthrobacter sp.
?
2-coumarate + NADH
-
390510
Arthrobacter sp.
melilotate + NAD+
-
390510
Arthrobacter sp.
ir
4-coumarate + NADH
1.4% of activity with 2-coumarate
390510
Arthrobacter sp.
?
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
8.5
o-coumarate + NADH
Arthrobacter sp.
Other publictions for EC 1.3.1.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
390510
Levy
The metabolism of coumarin by ...
Arthrobacter sp.
Biochemistry
3
1944-1947
1964
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