The metabolism of coumarin by a microorganism. II. The reduction of o-coumaric acid to melilotic acid

Levy, C.C.; Weinstein, G.D.; Biochemistry 3, 1944-1947 (1964)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

iodoacetate

3.3 mM, 29% inhibition

Arthrobacter sp.

N-ethylmaleimide

3.0 mM, 63% inhibition

Arthrobacter sp.

p-chloromercuribenzoate

3.0 mM, 100% inhibition, 0.33 mM, 37% inhibition

Arthrobacter sp.

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.0173

-

o-coumarate

-

Arthrobacter sp.

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

2-coumarate + NADH

Arthrobacter sp.

-

melilotate + NAD+

-

Arthrobacter sp.

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

2,4-dihydroxycinnamic acid + NADH

1.6% of activity with 2-coumarate

390510

Arthrobacter sp.

?

-

-

-

?

2-coumarate + NADH

-

390510

Arthrobacter sp.

melilotate + NAD+

-

390510

Arthrobacter sp.

?

2-coumarate + NADH

-

390510

Arthrobacter sp.

melilotate + NAD+

-

390510

Arthrobacter sp.

ir

4-coumarate + NADH

1.4% of activity with 2-coumarate

390510

Arthrobacter sp.

?

-

-

-

?

Cofactor

Commentary

Organism

Structure

NAD+

-

Arthrobacter sp.

Cofactor

Commentary

Organism

Structure

NAD+

-

Arthrobacter sp.

Inhibitors

Commentary

Organism

Structure

iodoacetate

3.3 mM, 29% inhibition

Arthrobacter sp.

N-ethylmaleimide

3.0 mM, 63% inhibition

Arthrobacter sp.

p-chloromercuribenzoate

3.0 mM, 100% inhibition, 0.33 mM, 37% inhibition

Arthrobacter sp.

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.0173

-

o-coumarate

-

Arthrobacter sp.

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

2-coumarate + NADH

Arthrobacter sp.

-

melilotate + NAD+

-

Arthrobacter sp.

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

2,4-dihydroxycinnamic acid + NADH

1.6% of activity with 2-coumarate

390510

Arthrobacter sp.

?

-

-

-

?

2-coumarate + NADH

-

390510

Arthrobacter sp.

melilotate + NAD+

-

390510

Arthrobacter sp.

?

2-coumarate + NADH

-

390510

Arthrobacter sp.

melilotate + NAD+

-

390510

Arthrobacter sp.

ir

4-coumarate + NADH

1.4% of activity with 2-coumarate

390510

Arthrobacter sp.

?

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

390510

Levy

The metabolism of coumarin by ...

Arthrobacter sp.

Biochemistry

3

1944-1947

1964

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