Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Clostridioides difficile |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M calcium acetate, 14 % (w/v) PEG 400, 0.1 M MES, pH 6.5 | Clostridioides difficile |
Protein Variants | Comment | Organism |
---|---|---|
D345A | the mutant shows reduced activity compared to the wild type enzyme | Clostridioides difficile |
D345N | the mutant shows reduced activity compared to the wild type enzyme | Clostridioides difficile |
E198Q | inactive | Clostridioides difficile |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin [iron-sulfur] cluster | Clostridioides difficile | - |
(E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin [iron-sulfur] cluster | Clostridioides difficile DSM 1296 | - |
(E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridioides difficile | A0A125V4E7 | cf. 1.3.8.1 | - |
Clostridioides difficile DSM 1296 | A0A125V4E7 | cf. 1.3.8.1 | - |
Purification (Comment) | Organism |
---|---|
Strep tag II column chromatography and Superdex 200 gel filtration | Clostridioides difficile |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin [iron-sulfur] cluster | - |
Clostridioides difficile | (E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin [iron-sulfur] cluster | - |
Clostridioides difficile DSM 1296 | (E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? |
Synonyms | Comment | Organism |
---|---|---|
electron transferring flavoprotein/butyryl-CoA dehydrogenase | - |
Clostridioides difficile |
EtfAB/Bcd | - |
Clostridioides difficile |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Clostridioides difficile | |
Ferredoxin | - |
Clostridioides difficile | |
NAD+ | - |
Clostridioides difficile |