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Literature summary for 1.3.1.109 extracted from

  • Aboulnaga, e.l.-.H.; Pinkenburg, O.; Schiffels, J.; El-Refai, A.; Buckel, W.; Selmer, T.
    Effect of an Oxygen-Tolerant Bifurcating Butyryl Coenzyme A Dehydrogenase/Electron-Transferring Flavoprotein Complex from Clostridium difficile on Butyrate Production in Escherichia coli (2013), J. Bacteriol., 195, 3704-3713.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.109

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Clostridioides difficile

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 butanoyl-CoA pH 7.5, 22-25°C Clostridioides difficile

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster Clostridioides difficile
-
 butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster
-
 ?
(E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster Clostridioides difficile DSM 1296T
-
 butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridioides difficile Q18AQ1 and Q18AQ6 and Q18AQ5 Q18AQ1: Bcd2 protein, Q18AQ6: electron transfer flavoproteins subunit beta, Q18AQ5: electron transfer flavoprotein subunit alpha
-
Clostridioides difficile DSM 1296T Q18AQ1 and Q18AQ6 and Q18AQ5 Q18AQ1: Bcd2 protein, Q18AQ6: electron transfer flavoproteins subunit beta, Q18AQ5: electron transfer flavoprotein subunit alpha
-

Oxidation Stability

Oxidation Stability Organism
oxygen-stable Clostridioides difficile

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridioides difficile

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster
-
 Clostridioides difficile butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster
-
 ?
(E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster
-
 Clostridioides difficile DSM 1296T butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster
-
 ?
(E)-but-2-enoyl-CoA + 2 NADH + oxidized ferredoxin iron-sulfur cluster the bifurcating butyryl-CoA dehydrogenase catalyzes the NADH-dependent reduction of ferredoxin coupled to the reduction of crotonyl-CoA also by NADH Clostridioides difficile butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster
-
 ?
(E)-but-2-enoyl-CoA + 2 NADH + oxidized ferredoxin iron-sulfur cluster the bifurcating butyryl-CoA dehydrogenase catalyzes the NADH-dependent reduction of ferredoxin coupled to the reduction of crotonyl-CoA also by NADH Clostridioides difficile DSM 1296T butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster
-
 ?
butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster
-
 Clostridioides difficile (E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster
-
 ?
butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster
-
 Clostridioides difficile DSM 1296T (E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster
-
 ?

Subunits

Subunits Comment Organism
trimer the butanoyl-CoA dehydrogenase complex is composed of the dehydrogenase and two electron-transferring flavoprotein subunits Clostridioides difficile

Synonyms

Synonyms Comment Organism
bifurcating butyryl coenzyme A dehydrogenase/electron-transferring flavoprotein complex
-
 Clostridioides difficile

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23 25 assay at Clostridioides difficile

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 butanoyl-CoA pH 7.5, 22-25°C Clostridioides difficile

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Clostridioides difficile

General Information

General Information Comment Organism
metabolism bifurcating butyryl-CoA dehydrogenase affects the energy yield of butyrate fermentation in the clostridial metabolism Clostridioides difficile

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2800
-
 butanoyl-CoA pH 7.5, 22-25°C Clostridioides difficile