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Literature summary for 1.3.1.104 extracted from
- An efficient and economical assay to screen for triclosan binding to FabI (2016), J. Biomol. Screen., 21, 391-398 .
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | development of a simple thermal shift assay, which does not use ACP-linked substrates, to determine the binding ability of triclosan to the enzyme's active site | Staphylococcus aureus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Staphylococcus aureus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A95V | about 15% of wild-type activtiy, no inhibition by triclosan and no shift in melting temperature in presence of both triclosan and NADP+ | Staphylococcus aureus |
| F204S | no shift in melting temperature in presence of both triclosan and NADP+ | Staphylococcus aureus |
| I193S | no shift in melting temperature in presence of both triclosan and NADP+ | Staphylococcus aureus |
| M99T | mutant shows a similar shift in melting temperature in presence of both triclosan and NADP+ as wild-type | Staphylococcus aureus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| triclosan | 1.12 microM, 9% residual activity | Staphylococcus aureus |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | A0A0H3K7V7 | - |
- |
| Staphylococcus aureus Newman | A0A0H3K7V7 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.66 | - |
pH not specified in the publication, 25°C | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| crotonyl-[acyl-carrier-protein] + NADPH + H+ | - |
Staphylococcus aureus | butyryl-[acyl-carrier-protein] + NADP+ | - |
? | |
| crotonyl-[acyl-carrier-protein] + NADPH + H+ | - |
Staphylococcus aureus Newman | butyryl-[acyl-carrier-protein] + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30397, calculated, x * 30397, mass spectrometry | Staphylococcus aureus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FabI | - |
Staphylococcus aureus |
| NWMN_0881 | - |
Staphylococcus aureus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 39.4 | - |
mutant A95V, melting temperature | Staphylococcus aureus |
| 40.2 | - |
mutant M99T, melting temperature | Staphylococcus aureus |
| 40.5 | - |
wild-type, melting temperature | Staphylococcus aureus |
| 58.8 | - |
wild-type, melting temperature in presence of both triclosan and NADP+. Neither triclosan nor NADP+ alone increases the Tm value | Staphylococcus aureus |
| 60.2 | - |
mutant M99T, melting temperature in presence of both triclosan and NADP+ | Staphylococcus aureus |
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