Literature summary for 1.3.1.103 extracted from

Kurata, A.; Fujita, M.; Mowafy, A.M.; Kamachi, H.; Kurihara, T.; Esaki, N.
Production of (S)-2-chloropropionate by asymmetric reduction of 2-chloroacrylate with 2-haloacrylate reductase coupled with glucose dehydrogenase (2008), J. Biosci. Bioeng., 105, 429-431.

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Application

Application	Comment	Organism
synthesis	construction of a system for asymmetric reduction of 2-chloroacrylate to produce (S)-2-chloropropionate with recombinant Escherichia coli cells producing 2-haloacrylate reductase from Burkholderia sp. WS and an NADPH regeneration system. The system provided 37.4 g/l (S)-2-chloropropionate in more than 99.9%e.e. (S)-2-Chloropropionate is a synthetic intermediate for phenoxypropionic acid herbicides	Burkholderia sp.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Burkholderia sp.

Organism

Organism	UniProt	Comment	Textmining
Burkholderia sp.	Q59I44	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-chloroacrylate + NADPH + H+	no activity with NADH	Burkholderia sp.	(S)-2-chloropropanoate + NADP+	-	?

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Release 2023.1 (January 2023)