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Literature summary for 1.3.1.103 extracted from

  • Kurata, A.; Kurihara, T.; Kamachi, H.; Esaki N.
    2-Haloacrylate reductase, a novel enzyme of the medium chain dehydrogenase/reductase superfamily that catalyzes the reduction of a carbon-carbon double bond of unsaturated organohalogen compounds (2005), J. Biol. Chem., 280, 20286-20291.
    View publication on PubMed
Search for more literature for 1.3.1.103

Application

Application Comment Organism
synthesis the enzyme is useful for the production of (S)-2-chloropropionate, which is used for the industrial production of aryloxyphenoxypropionic acid herbicides Burkholderia sp.

Cloned(Commentary)

Cloned (Comment) Organism
overproduced in Escherichia coli Burkholderia sp.

Inhibitors

Inhibitors Comment Organism Structure
2-bromoacrylate above 2.5 mM Burkholderia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 NADPH pH 8.0, 35°C Burkholderia sp.
0.19
-
 2-bromoacrylate pH 8.0, 35°C Burkholderia sp.
0.45
-
 2-chloroacrylate pH 8.0, 35°C Burkholderia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 gel filtration Burkholderia sp.
35656
-
 1 * 35656, calculated from sequence Burkholderia sp.
35660
-
 mass spectrometric analysis Burkholderia sp.
37000
-
 1 * 37000, SDS-PAGE Burkholderia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-bromoacrylate + NADPH + H+ Burkholderia sp. no activity with NADH (S)-2-bromopropanoate + NADP+
-
 ?
2-chloroacrylate + NADPH + H+ Burkholderia sp. the enzyme is involved in the degradation of unsaturated organohalogen compounds (S)-2-chloropropanoate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Burkholderia sp. Q59I44
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Burkholderia sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.9
-
 2-chloroacrylate, pH 8.0, 35°C Burkholderia sp.
2.8
-
 2-bromoacrylate, pH 8.0, 35°C Burkholderia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-bromoacrylate + NADPH + H+ no activity with NADH Burkholderia sp. (S)-2-bromopropanoate + NADP+
-
 ?
2-chloroacrylate + NADPH + H+ no activity with NADH Burkholderia sp. (S)-2-chloropropanoate + NADP+
-
 ?
2-chloroacrylate + NADPH + H+ the enzyme is involved in the degradation of unsaturated organohalogen compounds Burkholderia sp. (S)-2-chloropropanoate + NADP+
-
 ?
additional information no activity with 1,4-benzoquinone, 1,4-naphthoquinone, acrylate and methacrylate Burkholderia sp. ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 37000, SDS-PAGE Burkholderia sp.
monomer 1 * 35656, calculated from sequence Burkholderia sp.

Synonyms

Synonyms Comment Organism
CAA43
-
 Burkholderia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 assay at Burkholderia sp.
40
-
-
 Burkholderia sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 stable below, 30 min Burkholderia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Burkholderia sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 8 30°C, 30 min Burkholderia sp.

Cofactor

Cofactor Comment Organism Structure
NADPH no activity with NADH Burkholderia sp.