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Literature summary for 1.3.1.102 extracted from

  • Hirata, T.; Tamura, Y.; Yokobatake, N.; Shimoda, K.; Ashida, Y.
    A 38 kDa allylic alcohol dehydrogenase from the cultured cells of Nicotiana tabacum (2000), Phytochemistry, 55, 297-303.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information the Km-values for the (S)-alcohols are one order of magnitude lower than those for the (R)-alcohols, indicating that the enzyme has a higher affinity for the S-configuration than for the R-configuration Nicotiana tabacum
1.9
-
(2S,4R)-carveol pH 8.0, 35°C, NADP+ Nicotiana tabacum
2.5
-
(2S,4S)-carveol pH 8.0, 35°C, NADP+ Nicotiana tabacum
38
-
(2R,4R)-carveol pH 8.0, 35°C, NADP+ Nicotiana tabacum
52
-
(2R,4S)-carveol pH 8.0, 35°C, NADP+ Nicotiana tabacum
590
-
(1R,2S,4S)-neoisodihydrocarveol pH 8.0, 35°C, NADP+ Nicotiana tabacum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
alpha2, 2 * 37000, determined by SDS-PAGE Nicotiana tabacum
38080
-
allyl-ADH1, calculated from sequence Nicotiana tabacum
74000
-
approximately, gel filtration chromatography on a TSK G3000SW HPLC column Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum Q9SLN8
-
-

Purification (Commentary)

Purification (Comment) Organism
by DEAE-Toyopearl anion exchange column chromatography, followed by Red-Toyopearl affinity column chromatography Nicotiana tabacum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.11
-
Red-Toyopearl affinity column chromatography, pH 8.0, 35°C Nicotiana tabacum
4.6
-
DEAE-Toyopearl anion exchange column chromatography, pH 8.0, 35°C Nicotiana tabacum
5300
-
crude extract, pH 8.0, 35°C Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1R,2S,4S)-neoisodihydrocarveol + NADP+
-
Nicotiana tabacum (1R,4S)-isodihydrocarvone + NADPH + H+
-
r
(2R,4R)-carveol + NADP+
-
Nicotiana tabacum (R)-carvone + NADPH + H+
-
r
(2R,4S)-carveol + NADP+
-
Nicotiana tabacum (S)-carvone + NADPH + H+
-
r
(2S,4R)-carveol + NADP+
-
Nicotiana tabacum (R)-carvone + NADPH + H+
-
r
(2S,4S)-carveol + NADP+
-
Nicotiana tabacum (S)-carvone + NADPH + H+
-
r
additional information results indicate that the enzyme catalyzes specifically the dehydrogenation of the secondary alcohols adjacent to the C-C double bond, no oxidation of saturated alcohols Nicotiana tabacum ?
-
?
additional information the enzymatic reaction is a reversible oxidoreduction and the reaction is enantioselective Nicotiana tabacum ?
-
?

Subunits

Subunits Comment Organism
homodimer alpha2, 2 * 37000, determined by SDS-PAGE Nicotiana tabacum

Synonyms

Synonyms Comment Organism
allyl-ADH
-
Nicotiana tabacum
allyl-ADH1 the amino acid sequences of the deduced allyl-ADHs are different in four amino acid residues Nicotiana tabacum
allyl-ADH2 the amino acid sequences of the deduced allyl-ADHs are different in four amino acid residues, allyl-ADH2 has the following mutations compared to ADH1: T17A, V30A, L345P,R278H Nicotiana tabacum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.003
-
(1R,2S,4S)-neoisodihydrocarveol dehydrogenation, pH 8.0, 35°C, NADP+ Nicotiana tabacum
0.003
-
(1R,4S)-isodihydrocarvone hydrogenation to (1R,2S,4S)-neoisodihydrocarveol, pH 7.4, 35°C, NADP+ Nicotiana tabacum
0.01
-
(S)-carvone hydrogenation to (2R,4S)-carveol, pH 7.4, 35°C, NADP+ Nicotiana tabacum
0.012
-
(R)-carvone hydrogenation to (2R,4R)-carveol, pH 7.4, 35°C, NADP+ Nicotiana tabacum
0.02
-
(2R,4R)-carveol dehydrogenation, pH 8.0, 35°C, NADP+ Nicotiana tabacum
0.025
-
(2R,4S)-carveol dehydrogenation, pH 8.0, 35°C, NADP+ Nicotiana tabacum
0.025
-
(S)-carvone hydrogenation to (2S,4S)-carveol, pH 7.4, 35°C, NADP+ Nicotiana tabacum
0.032
-
(R)-carvone hydrogenation to (2S,4R)-carveol, pH 7.4, 35°C, NADP+ Nicotiana tabacum
0.077
-
(2S,4S)-carveol dehydrogenation, pH 8.0, 35°C, NADP+ Nicotiana tabacum
0.097
-
(2S,4R)-carveol dehydrogenation, pH 8.0, 35°C, NADP+ Nicotiana tabacum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
reduction of the carbonyl group of (S)-carvone Nicotiana tabacum
8
-
dehydrogenation of (2S,4S)-carveol Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
NAD+ utilization of NADP+ by the enzyme is four times greater than for NAD+ Nicotiana tabacum
NADP+ utilization of NADP+ by the enzyme is four times greater than for NAD+ Nicotiana tabacum