BRENDA - Enzyme Database
show all sequences of 1.3.1.101

Biosynthesis of archaeal membrane lipids: digeranylgeranylglycerophospholipid reductase of the thermoacidophilic archaeon Thermoplasma acidophilum

Nishimura, Y.; Eguchi, T.; J. Biochem. 139, 1073-1081 (2006)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
EDTA has little effect on activity
Thermoplasma acidophilum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane associated protein
Thermoplasma acidophilum
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
the enzyme does not require metal ions for activity
Thermoplasma acidophilum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
x * 45000, SDS-PAGE
Thermoplasma acidophilum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2,3-bis-O-geranylgeranyl-sn-glycerol 1-phosphate + 8 NAD(P)H + 8 H+
Thermoplasma acidophilum
formation of 2,3-di-O-phytanyl-sn-glyceryl phosphate (archaetidic acid), which is the basic core structure of archaeal membrane lipids
2,3-bis-O-phytanyl-sn-glycerol 1-phosphate + 8 NAD(P)+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Thermoplasma acidophilum
Q9HKS9
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Thermoplasma acidophilum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0115
-
pH 6.5, 50°C, substrate: 2,3-di-O-geranylgeranyl-sn-glycerol 1-phosphate
Thermoplasma acidophilum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2,3-bis-O-geranylgeranyl-sn-glycerol 1-phosphate + 8 NAD(P)H + 8 H+
-
719758
Thermoplasma acidophilum
2,3-bis-O-phytanyl-sn-glycerol 1-phosphate + 8 NAD(P)+
-
-
-
?
2,3-bis-O-geranylgeranyl-sn-glycerol 1-phosphate + 8 NAD(P)H + 8 H+
formation of 2,3-di-O-phytanyl-sn-glyceryl phosphate (archaetidic acid), which is the basic core structure of archaeal membrane lipids
719758
Thermoplasma acidophilum
2,3-bis-O-phytanyl-sn-glycerol 1-phosphate + 8 NAD(P)+
-
-
-
?
2,3-bis-O-phytyl-sn-glycero-phosphoethanolamine + NAD(P)H + H+
-
719758
Thermoplasma acidophilum
?
-
-
-
?
2,3-bis-O-phytyl-sn-glyceryl phosphate + NAD(P)H + H+
-
719758
Thermoplasma acidophilum
2,3-bis-O-phytanyl-sn-glycerol 1-phosphate + NAD(P)+
-
-
-
?
3-O-(2,3-bis-O-phytyl-sn-glycero-phospho)-sn-glycerol + NAD(P)H + H+
-
719758
Thermoplasma acidophilum
?
-
-
-
?
additional information
neither 2,3-bis-O-geranylgeranylglycerol nor 2,3-bis-O-geranylgeranylglyceryl phosphate dimethyl ester is recognized as a substrate
719758
Thermoplasma acidophilum
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 45000, SDS-PAGE
Thermoplasma acidophilum
Synonyms
Synonyms
Commentary
Organism
Ta0516m
-
Thermoplasma acidophilum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
aassay at
Thermoplasma acidophilum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
assay at
Thermoplasma acidophilum
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
required for activity
Thermoplasma acidophilum
NAD(P)H
required for activity
Thermoplasma acidophilum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
required for activity
Thermoplasma acidophilum
NAD(P)H
required for activity
Thermoplasma acidophilum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
EDTA has little effect on activity
Thermoplasma acidophilum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane associated protein
Thermoplasma acidophilum
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
the enzyme does not require metal ions for activity
Thermoplasma acidophilum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
x * 45000, SDS-PAGE
Thermoplasma acidophilum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2,3-bis-O-geranylgeranyl-sn-glycerol 1-phosphate + 8 NAD(P)H + 8 H+
Thermoplasma acidophilum
formation of 2,3-di-O-phytanyl-sn-glyceryl phosphate (archaetidic acid), which is the basic core structure of archaeal membrane lipids
2,3-bis-O-phytanyl-sn-glycerol 1-phosphate + 8 NAD(P)+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermoplasma acidophilum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0115
-
pH 6.5, 50°C, substrate: 2,3-di-O-geranylgeranyl-sn-glycerol 1-phosphate
Thermoplasma acidophilum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2,3-bis-O-geranylgeranyl-sn-glycerol 1-phosphate + 8 NAD(P)H + 8 H+
-
719758
Thermoplasma acidophilum
2,3-bis-O-phytanyl-sn-glycerol 1-phosphate + 8 NAD(P)+
-
-
-
?
2,3-bis-O-geranylgeranyl-sn-glycerol 1-phosphate + 8 NAD(P)H + 8 H+
formation of 2,3-di-O-phytanyl-sn-glyceryl phosphate (archaetidic acid), which is the basic core structure of archaeal membrane lipids
719758
Thermoplasma acidophilum
2,3-bis-O-phytanyl-sn-glycerol 1-phosphate + 8 NAD(P)+
-
-
-
?
2,3-bis-O-phytyl-sn-glycero-phosphoethanolamine + NAD(P)H + H+
-
719758
Thermoplasma acidophilum
?
-
-
-
?
2,3-bis-O-phytyl-sn-glyceryl phosphate + NAD(P)H + H+
-
719758
Thermoplasma acidophilum
2,3-bis-O-phytanyl-sn-glycerol 1-phosphate + NAD(P)+
-
-
-
?
3-O-(2,3-bis-O-phytyl-sn-glycero-phospho)-sn-glycerol + NAD(P)H + H+
-
719758
Thermoplasma acidophilum
?
-
-
-
?
additional information
neither 2,3-bis-O-geranylgeranylglycerol nor 2,3-bis-O-geranylgeranylglyceryl phosphate dimethyl ester is recognized as a substrate
719758
Thermoplasma acidophilum
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 45000, SDS-PAGE
Thermoplasma acidophilum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
aassay at
Thermoplasma acidophilum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
assay at
Thermoplasma acidophilum
Other publictions for EC 1.3.1.101
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
712772
Xu
Insights into substrate specif ...
Thermoplasma acidophilum
J. Mol. Biol.
404
403-417
2010
-
-
1
1
-
-
-
-
-
-
-
1
-
6
-
-
1
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
719041
Nishimura
Stereochemistry of reduction i ...
Thermoplasma acidophilum
Bioorg. Chem.
35
276-283
2007
-
-
1
-
-
-
-
-
1
-
1
1
-
2
-
-
1
-
-
-
-
-
2
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719758
Nishimura
Biosynthesis of archaeal membr ...
Thermoplasma acidophilum
J. Biochem.
139
1073-1081
2006
-
-
-
-
-
-
1
-
3
1
1
1
-
3
-
-
1
-
-
-
1
-
6
1
1
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
-
3
1
1
1
-
-
-
1
-
-
1
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-