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Literature summary for 1.3.1.10 extracted from

  • Xu, H.; Sullivan, T.J.; Sekiguchi, J.; Kirikae, T.; Ojima, I.; Stratton, C.F.; Mao, W.; Rock, F.L.; Alley, M.R.; Johnson, F.; Walker, S.G.; Tonge, P.J.
    Mechanism and inhibition of saFabI, the enoyl reductase from Staphylococcus aureus (2008), Biochemistry, 47, 4228-4236.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
A95V the mutant correlates with resistance to diphenyl ethers and causes a significant reduction in the affinity of the inhibitors for the enzyme Staphylococcus aureus
F204S inactive, the mutant correlates with resistance to diphenyl ethers and causes a significant reduction in the affinity of the inhibitors for the enzyme Staphylococcus aureus
I193S the mutant correlates with resistance to diphenyl ethers and causes a significant reduction in the affinity of the inhibitors for the enzyme Staphylococcus aureus
K41N strongly decreased activity for NADPH and increased activity with NADH Staphylococcus aureus
R40Q strongly decreased activity for NADPH and increased activity with NADH Staphylococcus aureus
R40Q/K41N strongly decreased activity for NADPH and increased activity with NADH Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
5-chloro-2-phenoxyphenol
-
Staphylococcus aureus
5-ethyl-2-phenoxyphenol
-
Staphylococcus aureus
triclosan
-
Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
dodecenoyl-[Staphylococcus aureus acyl carrier protein] wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
0.008
-
Crotonyl-N-acetyl-cysteamine wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
0.0114
-
dodecenoyl-[Staphylococcus aureus acyl carrier protein] mutant enzyme A95V, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
0.0115
-
crotonyl-[Staphylococcus aureus acyl carrier protein] wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
0.0184
-
dodecenoyl-[Staphylococcus aureus acyl carrier protein] mutant enzyme F204S, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
0.0233
-
dodecenoyl-N-acetyl-cysteamine wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
0.0241
-
dodec-2-enoyl-CoA wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
0.2694
-
NADPH mutant enzyme A95V, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
0.4296
-
NADPH mutant enzyme F204S, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
1
-
NADPH Km above 1 mM, mutant enzyme I193S, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
SDS-PAGE Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA resin chromatography Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
crotonyl-N-acetyl-cysteamine + NADPH + H+
-
Staphylococcus aureus butyryl-N-acetyl-cysteamine + NADP+
-
?
crotonyl-[Staphylococcus aureus acyl carrier protein] + NADPH + H+
-
Staphylococcus aureus butyryl-[Staphylococcus aureus acyl carrier protein] + NADP+
-
?
dodec-2-enoyl-CoA + NADPH + H+
-
Staphylococcus aureus dodecanoyl-CoA + NADP+
-
r
dodecenoyl-CoA + NADPH + H+
-
Staphylococcus aureus dodecanoyl-CoA + NADP+
-
?
dodecenoyl-N-acetyl-cysteamine + NADPH + H+
-
Staphylococcus aureus dodecanoyl-N-acetyl-cysteamine + NADP+
-
?
dodecenoyl-[Staphylococcus aureus acyl carrier protein] + NADPH + H+
-
Staphylococcus aureus dodecanoyl-[Staphylococcus aureus acyl carrier protein] + NADP+
-
?
additional information no activity with crotonyl-CoA and NADH Staphylococcus aureus ?
-
?
trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH + H+ prefers acyl carrier protein substrates carrying fatty acids with long acyl chains Staphylococcus aureus acyl-[acyl-carrier protein] + NADP+
-
?

Synonyms

Synonyms Comment Organism
enoyl reductase
-
Staphylococcus aureus
FabI
-
Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
Crotonyl-N-acetyl-cysteamine wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
11.6
-
crotonyl-[Staphylococcus aureus acyl carrier protein] wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
18
-
dodec-2-enoyl-CoA wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
18.4
-
dodecenoyl-[Staphylococcus aureus acyl carrier protein] mutant enzyme A95V, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
30.4
-
dodecenoyl-N-acetyl-cysteamine wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
130.2
-
NADPH wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
130.2
-
dodecenoyl-[Staphylococcus aureus acyl carrier protein] wild type enzyme, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus
157.3
-
dodecenoyl-[Staphylococcus aureus acyl carrier protein] mutant enzyme F204S, at 25°C, in 100 mM Na2HPO4 buffer (pH 7.8) Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
NADPH dependent Staphylococcus aureus