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Literature summary for 1.23.5.1 extracted from

  • Havir, E.A.; Tausta, S.L.; Peterson, R.B.
    Purification and properties of violaxanthin de-epoxidase from spinach (1997), Plant Sci., 123, 57-66.
No PubMed abstract available
Search for more literature for 1.23.5.1

Inhibitors

Inhibitors Comment Organism Structure
Cys 2.7 mM, 50% inhibition Spinacia oleracea
dithiothreitol 0.055 mM, 50% inhibition. The rate of conversion of violaxanthin to antheraxanthin is relatively unchanged whereas the conversion of antheraxanthin to zeaxanthin is 50-80% inhibited Spinacia oleracea
mercaptoethanol 0.68 mM, 50% inhibition Spinacia oleracea
o-phenanthroline 0.025 mM, 50% inhibition. The rate of conversion of violaxanthin to antheraxanthin is relatively unchanged whereas the conversion of antheraxanthin to zeaxanthin is 50-80% inhibited Spinacia oleracea
zeaxanthin product inhibition Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 violaxanthin
-
 Spinacia oleracea
0.0053
-
 antheraxanthin
-
 Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
thylakoid
-
 Spinacia oleracea 9579
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43300
-
 1 * 43300, SDS-PAGE Spinacia oleracea
45800
-
 gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
antheraxanthin + ascorbate Spinacia oleracea
-
 zeaxanthin + dehydroascorbate + H2O
-
 ?
violaxanthin + ascorbate Spinacia oleracea
-
 antheraxanthin + dehydroascorbate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
antheraxanthin + ascorbate
-
 Spinacia oleracea zeaxanthin + dehydroascorbate + H2O
-
 ?
violaxanthin + ascorbate
-
 Spinacia oleracea antheraxanthin + dehydroascorbate + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 43300, SDS-PAGE Spinacia oleracea

Synonyms

Synonyms Comment Organism
VDE
-
 Spinacia oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
-
 Spinacia oleracea

pI Value

Organism Comment pI Value Maximum pI Value
Spinacia oleracea isoelectric focusing
-
 4.95