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Literature summary for 1.23.5.1 extracted from
- Violaxanthin de-epoxidase. Lipid composition and substrate specificity (1978), Arch. Biochem. Biophys., 190, 514-522.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| monogalactosyldiacylglycerol | required for an active enzyme. Optimal ratio of monogalactosyldiacylglycerol to the substrate violaxanthin is 28:1 | Lactuca sativa |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000049 | - |
violaxanthin | pH 5.1, 26°C, at the optimal ratio of monogalactosyldiglyceride to the substrate violaxanthin is 28:1 | Lactuca sativa | |
| 0.00145 | - |
violaxanthin | pH 5.1, 26°C, at an ratio of monogalactosyldiacylglyceride to the substrate violaxanthin is 45:1 | Lactuca sativa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Lactuca sativa | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
gel filtration | Lactuca sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactuca sativa | - |
var. Romaine | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| antheraxanthin + ascorbate | the activity is 5.25fold higher than the activity of violaxanthin | Lactuca sativa | zeaxanthin + dehydroascorbate + H2O | - |
? | |
| cryptoxanthin epoxide + ascorbate | 92% of the activity with violaxanthin | Lactuca sativa | ? | - |
? | |
| diadinoxanthin + ascorbate | the activity is 2.25fold higher than the activity of violaxanthin | Lactuca sativa | ? | - |
? | |
| lutein epoxide + ascorbate | the activity is 1.33fold higher than the activity of violaxanthin | Lactuca sativa | ? | - |
? | |
| additional information | the enzyme is inactive against monoepoxy and diepoxy beta-carotene, antheraxanthin-A and lutein epoxide | Lactuca sativa | ? | - |
? | |
| neoxanthin + ascorbate | 10% of the activity with violaxanthin | Lactuca sativa | ? | - |
? | |
| violaxanthin + ascorbate | - |
Lactuca sativa | antheraxanthin + dehydroascorbate + H2O | - |
? | |
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Release 2023.1 (January 2023)
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