Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0073 | - |
(-)-pinoresinol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
0.0126 | - |
(-)-pinoresinol | at pH 7.5 and 30°C | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9FVQ6 | ecotype Columbia | - |
Arabidopsis thaliana | Q9SVP6 | ecotype Columbia | - |
Purification (Comment) | Organism |
---|---|
His-Bind resin affinity column chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | transcript levels in root are higher than those in stem | Arabidopsis thaliana | - |
stem | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-pinoresinol + NADPH + H+ | - |
Arabidopsis thaliana | (-)-lariciresinol + NADP+ | - |
? | |
(-)-pinoresinol + NADPH + H+ | efficient substrate | Arabidopsis thaliana | (-)-lariciresinol + NADP+ | - |
? | |
additional information | the activity toward (+/-)-lariciresinol is 35times lower than that of pinoresinol | Arabidopsis thaliana | ? | - |
? | |
additional information | the enzyme shows no activity with (+)-pinoresinol and lariciresinol | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PrR1 | - |
Arabidopsis thaliana |
PrR2 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the enantiomeric control in lignan biosynthesis | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.17 | - |
(-)-pinoresinol | at pH 7.5 and 30°C | Arabidopsis thaliana | |
19 | - |
(-)-pinoresinol | at pH 7.5 and 30°C | Arabidopsis thaliana |