Literature summary for 1.23.1.2 extracted from

Corbin, C.; Drouet, S.; Mateljak, I.; Markulin, L.; Decourtil, C.; Renouard, S.; Lopez, T.; Doussot, J.; Lamblin, F.; Auguin, D.; Laine, E.; Fuss, E.; Hano, C.
Functional characterization of the pinoresinol-lariciresinol reductase-2 gene reveals its roles in yatein biosynthesis and flax defense response (2017), Planta, 246, 405-420 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene PLR2, recombinant expression of N-terminal and C-terminal fusions of LuPLR2 with EGFP or GUS in transgenic tobacco mesophyll cells, and LuPLR2 overexpression in transgenic flax plants, via transformation by Agrobacterium tumefaciens strain GV3101, quantitative RT-PCR expression analysis of LuPLR1 and LuPLR2 expressions in leaves and seeds, subcloning in Escherichia coli strain HB101. cis-Elements, responsible for gene expression in response to stress, are located in separate portions of the LuPLR2 promoter, presence of two MYB-binding sites. Quantitative RT-PCR expression analysis of LuPLR2 in transgenic seedlings, analysis of the transcriptional regulation of the LuPLR2 gene	Linum usitatissimum

Cloned (Comment)

Organism

gene PLR2, recombinant expression of N-terminal and C-terminal fusions of LuPLR2 with EGFP or GUS in transgenic tobacco mesophyll cells, and LuPLR2 overexpression in transgenic flax plants, via transformation by Agrobacterium tumefaciens strain GV3101, quantitative RT-PCR expression analysis of LuPLR1 and LuPLR2 expressions in leaves and seeds, subcloning in Escherichia coli strain HB101. cis-Elements, responsible for gene expression in response to stress, are located in separate portions of the LuPLR2 promoter, presence of two MYB-binding sites. Quantitative RT-PCR expression analysis of LuPLR2 in transgenic seedlings, analysis of the transcriptional regulation of the LuPLR2 gene

Linum usitatissimum

Protein Variants

Protein Variants	Comment	Organism
additional information	isozyme LuPLR2 overexpression in Linum usitatissimum plants causes yatein accumulation. The deletion of the region from -473 to -170, containing four putative-binding sites for the WRKY transcription factor involved in the response to wounding or elicitors, results in a complete loss of the response to both methyljasmonate treatment and wounding. A significant decrease in the LuPLR2 gene expression is caused by the deletion of the region from -826 to -473, which contains two putative two binding sites for the WRKY transcription factor, involved in the plant defense response, overview. The four regions, among known cis-motifs from plants, reveal the presence of two MYB-binding sites	Linum usitatissimum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell surface	-	Linum usitatissimum	9986	-
additional information	identification of subcellular location of LuPLR2-derived lignan biosynthesis transgenic tobacco mesophyll cells that express LuPLR2-eGFP fusion proteins, LuPLR2 gene expression analysis	Linum usitatissimum	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(+)-pinoresinol + NADPH + H+	Linum usitatissimum	-	(+)-lariciresinol + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Linum usitatissimum	E6Y2X0	cv. Barbara	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	especially young leaves	Linum usitatissimum	-
additional information	quantitative RT-PCR expression analysis of LuPLR1 and LuPLR2 expressions in leaves and seeds, steady-state level of LuPLR1 mRNA in leaf tissues. Spatiotemporal LuPLR2 gene expression pattern in relation to yatein biosynthesis. The LuPLR2 gene encoding the second PLR isoform is highly expressed in vegetative tissue with highest levels in young leaves and to a lesser extent in old leaves and stem	Linum usitatissimum	-
root	-	Linum usitatissimum	-
seed	low expression level of isozyme PLR2	Linum usitatissimum	-
stem	-	Linum usitatissimum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(+)-pinoresinol + NADPH + H+	-	Linum usitatissimum	(+)-lariciresinol + NADP+	-	?
additional information	(+)-pinoresinol is successively converted into (-+)-lariciresinol and further into (-)-secoisolariciresinol by bifunctional enzyme PrR2, cf. EC 1.23.1.1	Linum usitatissimum	?	-	?

Synonyms

Synonyms	Comment	Organism
bifunctional pinoresinol-lariciresinol reductase 2	UniProt	Linum usitatissimum
LuPLR2	-	Linum usitatissimum
More	cf. EC 1.23.1.1	Linum usitatissimum
pinoresinol-lariciresinol reductase-2	-	Linum usitatissimum
PLR2	-	Linum usitatissimum

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Linum usitatissimum

Expression

Organism	Comment	Expression
Linum usitatissimum	analysis of transcriptional regulation of the LuPLR2 gene from flax by a PCR walking strategy, overview	additional information
Linum usitatissimum	methyljasmonate triggers the expression of LuPLR2 leading to an over-accumulation of yatein, while salicylic acid fails to act strongly on the expression of this gene and yatein accumulation is not stimulated. LuPLR2 gene expression shows an increase in wounded plants, LuPLR2 gene expression is induced in wounded leaves compared to the control, cis-elements, responsible for gene expression in response to stress, are located in separate portions of the LuPLR2 promoter. LuPLR2 expression and yatein production are increased by methyl jasmonate and wounding	up

General Information

General Information	Comment	Organism
metabolism	the enzyme is involved in the biosynthetic pathway of lignans in flax Linum usitatissimum, overview	Linum usitatissimum
additional information	analysis of transcriptional regulation of the LuPLR2 gene from flax, overview. Spatiotemporal LuPLR2 gene expression pattern in relation to yatein biosynthesis	Linum usitatissimum
physiological function	LuPLR2 is involved in the early steps of (-)-secoisolariciresinol ((-)-SECO) biosynthesis and derived lignans (yatein, hinokinin, bursehernin, thujaplicatin, and matairesinol dimethyl ether) accumulated mainly in leaves. In vivo involvement of the LuPLR2 gene in the biosynthesis of (-)-yatein accumulated in flax leaves. The enzyme expression is correlated to yatein, a lignan, accumulation in plant leaves. Lignans are probably involved in plant defense mechanisms and given the presence of several cis-regulatory elements potentially involved in the plant defense response revealed by the in silico analysis of LuPLR2, presence of two MYB-binding sites in LuPLR2	Linum usitatissimum