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Literature summary for 1.23.1.1 extracted from

  • Nakatsubo, T.; Mizutani, M.; Suzuki, S.; Hattori, T.; Umezawa, T.
    Characterization of Arabidopsis thaliana pinoresinol reductase, a new type of enzyme involved in lignan biosynthesis (2008), J. Biol. Chem., 283, 15550-15557.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
(+)-pinoresinol at pH 7.5 and 30°C Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FVQ6 ecotype Columbia
-

Purification (Commentary)

Purification (Comment) Organism
His-Bind resin affinity column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
root transcript levels in root are higher than those in stem Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-pinoresinol + NADPH + H+ efficient substrate Arabidopsis thaliana (+)-lariciresinol + NADP+
-
?
additional information the activity toward (+/-)-lariciresinol is 35times lower than that of pinoresinol Arabidopsis thaliana ?
-
?

Synonyms

Synonyms Comment Organism
PrR1
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Arabidopsis thaliana

General Information

General Information Comment Organism
metabolism the enzyme is involved in the enantiomeric control in lignan biosynthesis Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
12.8
-
(+)-pinoresinol at pH 7.5 and 30°C Arabidopsis thaliana