Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Novosphingobium aromaticivorans |
expressed in Escherichia coli BL21(DE3) cells | Sphingobium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration | Sphingobium sp. |
36000 | - |
1 * 36000, recombinant enzyme, SDS-PAGE | Sphingobium sp. |
36837 | - |
1 * 36837, calculated from amino acid sequence | Sphingobium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Novosphingobium aromaticivorans | - |
- |
- |
Novosphingobium aromaticivorans DSM 12444 | - |
- |
- |
Sphingobium sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Novosphingobium aromaticivorans |
nickel affinity column chromatography | Sphingobium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
1 micromol/mg/min specific activity towards (+/-)-lariciresinol in the presence of NADPH, at pH 7.0 and 30°C | Sphingobium sp. |
additional information | - |
1 micromol/min/mg specific activity toward (+/-)-lariciresinol in the presence of NADPH, at pH 7.0 and 30°C | Novosphingobium aromaticivorans |
32 | - |
toward (+/-)-pinoresinol in the presence of NADPH, at pH 7.0 and 30°C | Novosphingobium aromaticivorans |
46 | - |
toward (+/-)-syringaresinol in the presence of NADPH, at pH 7.0 and 30°C | Sphingobium sp. |
61 | - |
toward (+/-)-syringaresinol in the presence of NADPH, at pH 7.0 and 30°C | Novosphingobium aromaticivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+/-)-pinoresinol + NADPH + H+ | - |
Novosphingobium aromaticivorans | (+/-)-lariciresinol + NADP+ | - |
? | |
(+/-)-pinoresinol + NADPH + H+ | - |
Sphingobium sp. | (+/-)-lariciresinol + NADP+ | - |
? | |
(+/-)-pinoresinol + NADPH + H+ | - |
Novosphingobium aromaticivorans DSM 12444 | (+/-)-lariciresinol + NADP+ | - |
? | |
(+/-)-syringaresinol + NADPH + H+ | - |
Novosphingobium aromaticivorans | 5,5'-dimethoxylariciresinol + NADP+ | - |
? | |
(+/-)-syringaresinol + NADPH + H+ | - |
Sphingobium sp. | 5,5'-dimethoxylariciresinol + NADP+ | - |
? | |
(+/-)-syringaresinol + NADPH + H+ | - |
Novosphingobium aromaticivorans DSM 12444 | 5,5'-dimethoxylariciresinol + NADP+ | - |
? | |
additional information | negligible activity with (+/-)-lariciresinol compared to (+/-)-pinoresinol | Novosphingobium aromaticivorans | ? | - |
? | |
additional information | negligible activity with (+/-)-lariciresinol compared to (+/-)-pinoresinol | Sphingobium sp. | ? | - |
? | |
additional information | negligible activity with (+/-)-lariciresinol compared to (+/-)-pinoresinol | Novosphingobium aromaticivorans DSM 12444 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000, recombinant enzyme, SDS-PAGE | Sphingobium sp. |
monomer | 1 * 36837, calculated from amino acid sequence | Sphingobium sp. |
Synonyms | Comment | Organism |
---|---|---|
pinoresinol/lariciresinol reductase | - |
Novosphingobium aromaticivorans |
pinoresinol/lariciresinol reductase | - |
Sphingobium sp. |
PinZ | - |
Novosphingobium aromaticivorans |
PinZ | - |
Sphingobium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Novosphingobium aromaticivorans |
45 | - |
- |
Sphingobium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Sphingobium sp. |
7.5 | 8.5 | - |
Novosphingobium aromaticivorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | activity is 15fold higher with NADPH than that in the presence of NADH, suggesting that NADPH is a preferred electron donor | Novosphingobium aromaticivorans | |
NADPH | activity is 7fold higher with NADPH than that in the presence of NADH, suggesting that NADPH is a preferred electron donor | Sphingobium sp. |