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Literature summary for 1.21.99.5 extracted from

  • Kunze, C.; Diekert, G.; Schubert, T.
    Subtle changes in the active site architecture untangled overlapping substrate ranges and mechanistic differences of two reductive dehalogenases (2017), FEBS J., 284, 3520-3535 .
    View publication on PubMed
Search for more literature for 1.21.99.5

Protein Variants

Protein Variants Comment Organism
R357E inactive Desulfitobacterium hafniense
R357K the mutant shows about 1% of wild type activity Desulfitobacterium hafniense
R357N the mutant shows about 10% of wild type activity Desulfitobacterium hafniense
R357Q the mutant shows about 70% of wild type activity Desulfitobacterium hafniense
Y298F inactive Desulfitobacterium hafniense
Y298H the mutant shows about 30% of wild type activity Desulfitobacterium hafniense
Y298S the mutant shows about 3% of wild type activity Desulfitobacterium hafniense
Y298T the mutant shows about 10% of wild type activity Desulfitobacterium hafniense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trichloroethene + chloride + acceptor Desulfitobacterium hafniense
-
 tetrachloroethene + reduced acceptor
-
 ?
trichloroethene + chloride + acceptor Desulfitobacterium hafniense Y51
-
 tetrachloroethene + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Desulfitobacterium hafniense
-
-
-
Desulfitobacterium hafniense Y51
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows no dihaloelimination activity towards 1,1,2-trichloroethane or 1,1,1-trichloroethane Desulfitobacterium hafniense ?
-
 ?
additional information the enzyme shows no dihaloelimination activity towards 1,1,2-trichloroethane or 1,1,1-trichloroethane Desulfitobacterium hafniense Y51 ?
-
 ?
trichloroethene + chloride + acceptor
-
 Desulfitobacterium hafniense tetrachloroethene + reduced acceptor
-
 ?
trichloroethene + chloride + acceptor
-
 Desulfitobacterium hafniense Y51 tetrachloroethene + reduced acceptor
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Desulfitobacterium hafniense

Synonyms

Synonyms Comment Organism
PCE reductive dehalogenase
-
 Desulfitobacterium hafniense
PceA
-
 Desulfitobacterium hafniense
tetrachloroethene reductive dehalogenase
-
 Desulfitobacterium hafniense