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Literature summary for 1.21.99.5 extracted from

  • Van de Pas, B.A.; Gerritse, J.; De Vos, W.M.; Schraa, G.; Stams, A.J.M.
    Two distinct enzyme systems are responsible for tetrachloroethene and chlorophenol reductive dehalogenation in Desulfitobacterium strain PCE1 (2001), Arch. Microbiol., 176, 165-169.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
N2O 23 mM: 25fold inhibition; dinitrogen oxide Desulfitobacterium hafniense
N2O 23 mM: 25fold inhibition; dinitrogen oxide Desulfitobacterium sp. PCE1
Propyl iodide 1-iodopropane Desulfitobacterium hafniense
Propyl iodide 0.05 mM 1-iodopropane: 3fold inhibition, completely reversed by light, light-reversible inhibition; 1-iodopropane Desulfitobacterium sp. PCE1

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasmic membrane membrane-bound Desulfitobacterium sp. PCE1
-
-
cytoplasmic membrane membrane-bound Desulfitobacterium hafniense
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cobalamin cobalamin-containing enzyme Desulfitobacterium sp. PCE1
Cobalamin cobalamin-containing enzyme Desulfitobacterium hafniense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
-
Desulfitobacterium sp. PCE1
59000
-
-
Desulfitobacterium hafniense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tetrachloroethene + reduced acceptor Desulfitobacterium sp. PCE1
-
trichloroethene + chloride + acceptor
-
?
tetrachloroethene + reduced acceptor Desulfitobacterium hafniense
-
trichloroethene + chloride + acceptor
-
?
tetrachloroethene + reduced acceptor Desulfitobacterium hafniense TCE1
-
trichloroethene + chloride + acceptor
-
?

Organism

Organism UniProt Comment Textmining
Desulfitobacterium hafniense
-
-
-
Desulfitobacterium hafniense TCE1
-
-
-
Desulfitobacterium sp. PCE1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Desulfitobacterium sp. PCE1
partial Desulfitobacterium hafniense

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Desulfitobacterium sp. PCE1
additional information
-
-
Desulfitobacterium hafniense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity Desulfitobacterium sp. PCE1 ?
-
?
additional information substrate specificity Desulfitobacterium hafniense ?
-
?
additional information no activity with 3-chloro-4-hydroxyphenylacetate and other ortho-chlorophenolic compounds, 2-chlorophenol, 3-chlorophenol, 4-chlorophenol, 2,3-dichlorophenol, 2,4-dichlorophenol, 2,5-dichlorophenol, 2,6-dichlorophenol, cis-dichloroethene Desulfitobacterium sp. PCE1 ?
-
?
additional information no activity with 3-chloro-4-hydroxyphenylacetate and other ortho-chlorophenolic compounds, 2-chlorophenol, 3-chlorophenol, 4-chlorophenol, 2,3-dichlorophenol, 2,4-dichlorophenol, 2,5-dichlorophenol, 2,6-dichlorophenol, cis-dichloroethene Desulfitobacterium hafniense ?
-
?
additional information substrate specificity Desulfitobacterium hafniense TCE1 ?
-
?
additional information no activity with 3-chloro-4-hydroxyphenylacetate and other ortho-chlorophenolic compounds, 2-chlorophenol, 3-chlorophenol, 4-chlorophenol, 2,3-dichlorophenol, 2,4-dichlorophenol, 2,5-dichlorophenol, 2,6-dichlorophenol, cis-dichloroethene Desulfitobacterium hafniense TCE1 ?
-
?
tetrachloroethene + reduced acceptor
-
Desulfitobacterium sp. PCE1 trichloroethene + chloride + acceptor
-
?
tetrachloroethene + reduced acceptor
-
Desulfitobacterium hafniense trichloroethene + chloride + acceptor
-
?
tetrachloroethene + reduced acceptor perchloroethylene, perchloroethene, PCE Desulfitobacterium sp. PCE1 trichloroethene + chloride + acceptor
-
?
tetrachloroethene + reduced acceptor perchloroethylene, perchloroethene, PCE Desulfitobacterium hafniense trichloroethene + chloride + acceptor
-
?
tetrachloroethene + reduced acceptor
-
Desulfitobacterium hafniense TCE1 trichloroethene + chloride + acceptor
-
?
tetrachloroethene + reduced acceptor perchloroethylene, perchloroethene, PCE Desulfitobacterium hafniense TCE1 trichloroethene + chloride + acceptor
-
?
trichloroethene + reduced acceptor poor substrate, 10% of the rate of tetrachloroethene Desulfitobacterium sp. PCE1 cis-1,2-dichloroethene + chloride + acceptor
-
?
trichloroethene + reduced acceptor good substrate, comparable to tetrachloroethene Desulfitobacterium hafniense cis-1,2-dichloroethene + chloride + acceptor
-
?
trichloroethene + reduced acceptor good substrate, comparable to tetrachloroethene Desulfitobacterium hafniense TCE1 cis-1,2-dichloroethene + chloride + acceptor
-
?

Subunits

Subunits Comment Organism
monomer 1 * 59000, SDS-PAGE Desulfitobacterium hafniense
monomer 1 * 48000, SDS-PAGE Desulfitobacterium sp. PCE1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Desulfitobacterium sp. PCE1
30
-
assay at Desulfitobacterium hafniense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Desulfitobacterium sp. PCE1
7.8
-
assay at Desulfitobacterium hafniense