Application | Comment | Organism |
---|---|---|
analysis | reductive dehalogenation assay can be used for the sensitive and rapid quantification of the corrinoid cofactor | Sulfurospirillum multivorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cis-1,3-dichloropropene | 50% inhibition | Sulfurospirillum multivorans | |
additional information | no inhibition by 1-chloropropene, 2-chloropropene, 3-chloropropene, 1,1-dichloropropene, 1,2-dichloropropane, 1,3-dichloropropane, and 1,2,3-trichloropropane | Sulfurospirillum multivorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | enzyme kinetics and kinetic parameters | Sulfurospirillum multivorans | |
0.2 | - |
tetrachloroethene | reduced 1.6 mM methyl viologen as electron donor | Sulfurospirillum multivorans | |
0.24 | - |
trichloroethene | reduced 1.6 mM methyl viologen as electron donor | Sulfurospirillum multivorans | |
0.25 | - |
trans-1,3-dichloropropene | reduced 1.6 mM methyl viologen as electron donor | Sulfurospirillum multivorans | |
0.25 | - |
1,1,3-trichloropropene | reduced 1.6 mM methyl viologen as electron donor | Sulfurospirillum multivorans | |
0.28 | - |
methyl viologen | at a tetrachloroethene concentration of 1 mM | Sulfurospirillum multivorans | |
0.6 | - |
2,3-dichloropropene | reduced 1.6 mM methyl viologen as electron donor | Sulfurospirillum multivorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | two Fe/S clusters | Sulfurospirillum multivorans | |
Iron-sulfur cluster | two Fe/S clusters | Sulfurospirillum multivorans | |
iron-sulfur clusters | two Fe/S clusters | Sulfurospirillum multivorans | |
S | two Fe/S clusters | Sulfurospirillum multivorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tetrachloroethene + reduced acceptor | Sulfurospirillum multivorans | - |
trichloroethene + chloride + acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurospirillum multivorans | - |
strictly anaerobic, gram-negative bacterium | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,1,3-trichloropropene + reduced acceptor | 22.9% activity compared with perchloroethene as substrate | Sulfurospirillum multivorans | 1,1-dichloropropene + chloride + acceptor | - |
? | |
2,3-dichloropropene + reduced acceptor | 14.4% activity compared with perchloroethene as substrate | Sulfurospirillum multivorans | 2-chloropropene + chloride + acceptor | - |
? | |
chlorinated propenes + reduced acceptor | 22% of the reduction rates with perchloroethene | Sulfurospirillum multivorans | propene + chloride + acceptor | - |
? | |
chloroacetamide + reduced acceptor | very poor substrate | Sulfurospirillum multivorans | acetamide + chloride + acceptor | - |
? | |
cis-1,3-dichloropropene + reduced acceptor | chlorination rate is lower than for trans-1,3-dichloropropene and reaction ceases after 2-3 min | Sulfurospirillum multivorans | mono-chloropropene + chloride + acceptor | - |
? | |
additional information | substrate specificity | Sulfurospirillum multivorans | ? | - |
? | |
additional information | no activity with dichloroethene, 1-chloropropene, 2-chloropropene, 3-chloropropene, 1,1-dichloropropene, 1,2-dichloropropane, 1,3-dichloropropane, and 1,2,3-trichloropropane | Sulfurospirillum multivorans | ? | - |
? | |
tetrachloroethene + reduced acceptor | - |
Sulfurospirillum multivorans | trichloroethene + chloride + acceptor | - |
? | |
tetrachloroethene + reduced acceptor | besides reduced methyl viologen, titanium(III)citrate can serve as electron donor for reductive dehalogenation | Sulfurospirillum multivorans | trichloroethene + chloride + acceptor | - |
? | |
tetrachloroethene + reduced acceptor | perchloroethylene, perchloroethene, PCE | Sulfurospirillum multivorans | trichloroethene + chloride + acceptor | - |
? | |
tetrachloromethane + reduced acceptor | very poor substrate | Sulfurospirillum multivorans | ? + chloride + acceptor | - |
? | |
trans-1,3-dichloropropene + reduced acceptor | 5.7% activity compared with perchloroethene as substrate | Sulfurospirillum multivorans | mono-chloropropene + chloride + acceptor | - |
? | |
trichloroethene + reduced acceptor | besides reduced methyl viologen, titanium(III)citrate can serve as electron donor for reductive dehalogenation | Sulfurospirillum multivorans | cis-1,2-dichloroethene + chloride + acceptor | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Sulfurospirillum multivorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
tetrachloroethene | titanium(III)citrate as sole electron donor | Sulfurospirillum multivorans | |
20.4 | - |
tetrachloroethene | methyl viologen as artificial electron donor | Sulfurospirillum multivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Sulfurospirillum multivorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
corrinoid | - |
Sulfurospirillum multivorans |