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Literature summary for 1.21.99.5 extracted from

  • Neumann, A.; Siebert, A.; Trescher, T.; Reinhardt, S.; Wohlfarth, G.; Diekert, G.
    Tetrachloroethene reductive dehalogenase of Dehalospirillum multivorans: substrate specificity of the native enzyme and its corrinoid cofactor (2002), Arch. Microbiol., 177, 420-426.
    View publication on PubMed
Search for more literature for 1.21.99.5

Application

Application Comment Organism
analysis reductive dehalogenation assay can be used for the sensitive and rapid quantification of the corrinoid cofactor Sulfurospirillum multivorans

Inhibitors

Inhibitors Comment Organism Structure
cis-1,3-dichloropropene 50% inhibition Sulfurospirillum multivorans
additional information no inhibition by 1-chloropropene, 2-chloropropene, 3-chloropropene, 1,1-dichloropropene, 1,2-dichloropropane, 1,3-dichloropropane, and 1,2,3-trichloropropane Sulfurospirillum multivorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information enzyme kinetics and kinetic parameters Sulfurospirillum multivorans
0.2
-
 tetrachloroethene reduced 1.6 mM methyl viologen as electron donor Sulfurospirillum multivorans
0.24
-
 trichloroethene reduced 1.6 mM methyl viologen as electron donor Sulfurospirillum multivorans
0.25
-
 trans-1,3-dichloropropene reduced 1.6 mM methyl viologen as electron donor Sulfurospirillum multivorans
0.25
-
 1,1,3-trichloropropene reduced 1.6 mM methyl viologen as electron donor Sulfurospirillum multivorans
0.28
-
 methyl viologen at a tetrachloroethene concentration of 1 mM Sulfurospirillum multivorans
0.6
-
 2,3-dichloropropene reduced 1.6 mM methyl viologen as electron donor Sulfurospirillum multivorans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ two Fe/S clusters Sulfurospirillum multivorans
Iron-sulfur cluster two Fe/S clusters Sulfurospirillum multivorans
iron-sulfur clusters two Fe/S clusters Sulfurospirillum multivorans
S two Fe/S clusters Sulfurospirillum multivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tetrachloroethene + reduced acceptor Sulfurospirillum multivorans
-
 trichloroethene + chloride + acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Sulfurospirillum multivorans
-
 strictly anaerobic, gram-negative bacterium
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,1,3-trichloropropene + reduced acceptor 22.9% activity compared with perchloroethene as substrate Sulfurospirillum multivorans 1,1-dichloropropene + chloride + acceptor
-
 ?
2,3-dichloropropene + reduced acceptor 14.4% activity compared with perchloroethene as substrate Sulfurospirillum multivorans 2-chloropropene + chloride + acceptor
-
 ?
chlorinated propenes + reduced acceptor 22% of the reduction rates with perchloroethene Sulfurospirillum multivorans propene + chloride + acceptor
-
 ?
chloroacetamide + reduced acceptor very poor substrate Sulfurospirillum multivorans acetamide + chloride + acceptor
-
 ?
cis-1,3-dichloropropene + reduced acceptor chlorination rate is lower than for trans-1,3-dichloropropene and reaction ceases after 2-3 min Sulfurospirillum multivorans mono-chloropropene + chloride + acceptor
-
 ?
additional information substrate specificity Sulfurospirillum multivorans ?
-
 ?
additional information no activity with dichloroethene, 1-chloropropene, 2-chloropropene, 3-chloropropene, 1,1-dichloropropene, 1,2-dichloropropane, 1,3-dichloropropane, and 1,2,3-trichloropropane Sulfurospirillum multivorans ?
-
 ?
tetrachloroethene + reduced acceptor
-
 Sulfurospirillum multivorans trichloroethene + chloride + acceptor
-
 ?
tetrachloroethene + reduced acceptor besides reduced methyl viologen, titanium(III)citrate can serve as electron donor for reductive dehalogenation Sulfurospirillum multivorans trichloroethene + chloride + acceptor
-
 ?
tetrachloroethene + reduced acceptor perchloroethylene, perchloroethene, PCE Sulfurospirillum multivorans trichloroethene + chloride + acceptor
-
 ?
tetrachloromethane + reduced acceptor very poor substrate Sulfurospirillum multivorans ? + chloride + acceptor
-
 ?
trans-1,3-dichloropropene + reduced acceptor 5.7% activity compared with perchloroethene as substrate Sulfurospirillum multivorans mono-chloropropene + chloride + acceptor
-
 ?
trichloroethene + reduced acceptor besides reduced methyl viologen, titanium(III)citrate can serve as electron donor for reductive dehalogenation Sulfurospirillum multivorans cis-1,2-dichloroethene + chloride + acceptor
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Sulfurospirillum multivorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
 tetrachloroethene titanium(III)citrate as sole electron donor Sulfurospirillum multivorans
20.4
-
 tetrachloroethene methyl viologen as artificial electron donor Sulfurospirillum multivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Sulfurospirillum multivorans

Cofactor

Cofactor Comment Organism Structure
corrinoid
-
 Sulfurospirillum multivorans