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Literature summary for 1.21.99.4 extracted from

  • Piehl, S.; Heberer, T.; Balizs, G.; Scanlan, T.S.; Kohrle, J.
    Development of a validated liquid chromatography/tandem mass spectrometry method for the distinction of thyronine and thyronamine constitutional isomers and for the identification of new deiodinase substrates (2008), Rapid Commun. Mass Spectrom., 22, 3286-3296.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
L-3,5',3'-triiodothyronine pH 7.4, 37°C, isozyme Dio2 Homo sapiens
0.57
-
L-3,5',3'-triiodothyronine pH 7.4, 37°C, isozyme Dio1 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Mus musculus 16020
-
membrane
-
Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus thyronines and thyronamines are two groups of endogenous iodine-containing signaling molecules that are substrates of three deiodinase isozymes, which catalyze the sequential reductive removal of iodine from the respective precursor molecule. Development of a analytical method applying liquid chromatography/tandem mass spectrometry, overview ?
-
?
additional information Homo sapiens thyronines and thyronamines are two groups of endogenous iodine-containing signaling molecules that are substrates of three deiodinase isozymes, which catalyze the sequential reductive removal of iodine from the respective precursor molecule. Development of a analytical method applying liquid chromatography/tandem mass spectrometry, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
three deiodinase isozymes Dio1, Dio2, and Dio3
-
Mus musculus
-
three deiodinase isozymes Dio1, Dio2, and Dio3
-

Source Tissue

Source Tissue Comment Organism Textmining
ECC-1 cell isozyme Dio3 Homo sapiens
-
Hep-G2 cell isozyme Dio1 Homo sapiens
-
liver
-
Mus musculus
-
liver
-
Homo sapiens
-
MSTO-211H cell isozyme Dio2 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-3,5',3'-triiodothyronine + AH2
-
Mus musculus L-3,3'-diiodothyronine + iodide + A + H+
-
?
L-3,5',3'-triiodothyronine + AH2 5'-deionination by isozyme Dio1 Homo sapiens L-3,3'-diiodothyronine + iodide + A + H+
-
?
L-3,5'-diiodothyonine + AH2
-
Mus musculus L-3-iodothyronine + iodide + A + H+
-
?
L-3,5'-diiodothyronine + AH2
-
Homo sapiens L-3-iodothyronine + iodide + A + H+
-
?
L-3,5,3',5'-tetraiodothyronine + AH2 i.e. thyroxine Mus musculus L-3,5,3'-triiodothyronine + iodide + A + H+
-
?
L-3,5,3',5'-tetraiodothyronine + AH2 i.e. thyroxine Homo sapiens L-3,5,3'-triiodothyronine + iodide + A + H+
-
?
L-3,5,3',5'-tetraiodothyronine + AH2 i.e. thyroxine Homo sapiens L-3,5,3'-triiodothyronine + iodide + A + H+ triiodothyrosine, a small 3,3'-T2 peak also occurs ?
additional information thyronines and thyronamines are two groups of endogenous iodine-containing signaling molecules that are substrates of three deiodinase isozymes, which catalyze the sequential reductive removal of iodine from the respective precursor molecule. Development of a analytical method applying liquid chromatography/tandem mass spectrometry, overview Mus musculus ?
-
?
additional information thyronines and thyronamines are two groups of endogenous iodine-containing signaling molecules that are substrates of three deiodinase isozymes, which catalyze the sequential reductive removal of iodine from the respective precursor molecule. Development of a analytical method applying liquid chromatography/tandem mass spectrometry, overview Homo sapiens ?
-
?
additional information isozyme Dio1 exhibits both phenolic and tyrosyl ring deiodination activity while isozyme Dio2 catalyzes only deiodinations of the phenolic ring, isozyme Dio3 catalyzes only deiodinations of the tyrosyl ring. T3, 3,3'-T2, and 3,5-T2 are also not deiodinated by Dio1 from HepG2 lysates Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
deiodinase
-
Mus musculus
deiodinase
-
Homo sapiens
Dio
-
Mus musculus
Dio
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Mus musculus
7.4
-
assay at Homo sapiens