Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | high concentrations of cofactor increases type I activity | Rattus sp. | |
reduced thiols | e.g. dithiothreitol | Rattus sp. |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli via phage lambda infection | Rattus sp. |
overexpression in rat astrocytes of 29 kD subunit with and without GFP-tag via adenovirus infection | Rattus sp. |
General Stability | Organism |
---|---|
very short lifetime in vivo of isoenzyme type II (D2) from brain | Rattus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | L-thyroxine inhibited competitively 3,3',5'-triiodothyronine utilization of isoenzyme type II | Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0067 | - |
3,3',5'-triiodothyronine | recombinant isoenzyme D2 (type II) from induced brain astrocytes with cosubstrate dithiothreitol KM: 18 mM | Rattus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound, type II integral in neurolemmal membranes | Rattus sp. | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
several subunits, thereof 1 * 29000 substrate binding subunit + 1 * 60000 cAMP-activation subunit + unknown number of other subunits, sequence analysis | Rattus sp. |
60000 | - |
several subunits, thereof 1 * 29000 substrate binding subunit + 1 * 60000 cAMP-activation subunit + unknown number of other subunits, sequence analysis | Rattus sp. |
200000 | - |
- |
Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,3',5'-triiodothyronine + AH2 | Rattus sp. | isoenzyme type I | 3,3'-diiodothyronine + iodide + A + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3,3',5-triiodo-L-thyronine + iodide + acceptor + H+ = L-thyroxine + reduced acceptor | type III, inner (tyrosyl) ring deiodination | Rattus sp. | |
3,3',5-triiodo-L-thyronine + iodide + acceptor + H+ = L-thyroxine + reduced acceptor | type II, outer ring (phenolic) deiodination | Rattus sp. | |
3,3',5-triiodo-L-thyronine + iodide + acceptor + H+ = L-thyroxine + reduced acceptor | type I, outer ring (phenolic) deiodination | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | isoenzyme D2 | Rattus sp. | - |
glial cell | i.e. brain astrocytes in culture | Rattus sp. | - |
glial cell | isoenzyme type II (D2) | Rattus sp. | - |
kidney | isoenzyme type I (D1) | Rattus sp. | - |
liver | isoenzyme typ I (D1) | Rattus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.2 | - |
isoenzyme D2 (type II) in cerebral cortex | Rattus sp. |
2 | - |
isoenzyme D2 (type II) induced in cultured astrocytes | Rattus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,3',5'-triiodothyronine + AH2 | isoenzyme type I | Rattus sp. | 3,3'-diiodothyronine + iodide + A + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | several subunits, thereof 1 * 29000 substrate binding subunit + 1 * 60000 cAMP-activation subunit + unknown number of other subunits, sequence analysis | Rattus sp. |
Synonyms | Comment | Organism |
---|---|---|
iodothyronine 5'-deiodinase | type II, no selenoprotein, induced by bt2cAMP and hydrocortisone (ED50 4nM) | Rattus sp. |
iodothyronine 5'-deiodinase | type I, selenoprotein, inducible by T3 (higher synthesis) and angiotensin II (positive chronotropic effect in myocard) | Rattus sp. |
iodothyronine 5'-deiodinase | isoenzymes type I (D1) and type II (D2) | Rattus sp. |