Activating Compound | Comment | Organism | Structure |
---|---|---|---|
12-O-tetradecanoylphorbol-13-acetate | induction of isozyme type III from brain astrocytes | Rattus norvegicus | |
acidic fibroblast growth factor | slight increase of 3,3',5,5'-tetraiodo-L-thyronine 5-deiodinase activity by isozyme type III | Rattus norvegicus | |
acidic fibroblast growth factor | induction of isozyme type III 3,3',5-triiodo-L-thyronine 5-deiodinase activity from brain astrocytes, 6fold higher in Se-repleted than in depleted cells | Rattus norvegicus | |
actinomycin D | induction of isozyme type III from brain astrocytes | Rattus norvegicus | |
cAMP | induction of isozyme type III from brain astrocytes | Rattus norvegicus | |
retinoic acid | induction of isozyme type III from brain astrocytes | Rattus norvegicus | |
Se2+ | required, depletion reversibly reduces the activity of isozyme type III by 3-10fold in cultured brain astrocytes | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aurothioglucose | no inhibition, isozyme type III | Rattus norvegicus | |
cycloheximide | isozyme type III, slight decrease of activity in vivo | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000001 | - |
3,3',5-triiodo-L-thyronine | pH 7.4, 37°C | Rattus norvegicus | |
0.000001 | - |
3,3',5-triiodo-L-thyronine | not influenced by selenium-deficiency | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
selenoprotein | - |
Rattus norvegicus | - |
isozyme type III | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
astrocyte | cell culture | Rattus norvegicus | - |
astrocyte | isozyme type III | Rattus norvegicus | - |
cell culture | astrocytes | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,3',5-triiodo-L-thyronine + AH2 | isozyme type III | Rattus norvegicus | 3,3'-diiodo-L-thyronine + iodide + A + H+ | - |
? | |
L-thyroxine + AH2 | preferred substrate | Rattus norvegicus | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | - |
? | |
L-thyroxine + AH2 | isozyme type III | Rattus norvegicus | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | - |
? | |
L-thyroxine + AH2 | 5-deiodinase activity | Rattus norvegicus | 3,3',5'-triiodo-L-thyronine + iodide + A + H+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |