Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | BchE activity is stimulated by increasing concentrations of NADPH or SAM | Rhodobacter capsulatus | |
NADPH | - |
Rhodobacter capsulatus | |
S-adenosyl-L-methionine | - |
Rhodobacter capsulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-homocysteine | - |
Rhodobacter capsulatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Rhodobacter capsulatus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Rhodobacter capsulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O | Rhodobacter capsulatus | overall reaction | 3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine | - |
? | |
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O | Rhodobacter capsulatus NBRC 16581 | overall reaction | 3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine | - |
? | |
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O | Rhodobacter capsulatus ATCC BAA-309 | overall reaction | 3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine | - |
? | |
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O | Rhodobacter capsulatus SB1003 | overall reaction | 3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | P26168 | - |
- |
Rhodobacter capsulatus ATCC BAA-309 | P26168 | - |
- |
Rhodobacter capsulatus NBRC 16581 | P26168 | - |
- |
Rhodobacter capsulatus SB1003 | P26168 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O = 3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine | the oxygen-independent conversion of Mg-protoporphyrin IX monomethyl ester (Mg-PME) to protochlorophyllide (Pchlide) is catalyzed by the anaerobic Mg-PME cyclase termed BchE, via an unusual radical S-adenosylmethionine (SAM) and cobalamin-dependent BchE catalysis | Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O | overall reaction | Rhodobacter capsulatus | 3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine | - |
? | |
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O | overall reaction | Rhodobacter capsulatus NBRC 16581 | 3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine | - |
? | |
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O | overall reaction | Rhodobacter capsulatus ATCC BAA-309 | 3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine | - |
? | |
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O | overall reaction | Rhodobacter capsulatus SB1003 | 3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine | - |
? | |
additional information | establishment of in vitro biosynthesis of the isocyclic ring moiety of bacteriochlorophyll using purified recombinant BchE, method development, overview | Rhodobacter capsulatus | ? | - |
- |
|
additional information | establishment of in vitro biosynthesis of the isocyclic ring moiety of bacteriochlorophyll using purified recombinant BchE, method development, overview | Rhodobacter capsulatus NBRC 16581 | ? | - |
- |
|
additional information | establishment of in vitro biosynthesis of the isocyclic ring moiety of bacteriochlorophyll using purified recombinant BchE, method development, overview | Rhodobacter capsulatus ATCC BAA-309 | ? | - |
- |
|
additional information | establishment of in vitro biosynthesis of the isocyclic ring moiety of bacteriochlorophyll using purified recombinant BchE, method development, overview | Rhodobacter capsulatus SB1003 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
BChE | - |
Rhodobacter capsulatus |
Mg-protoporphyrin IX monomethyl ester cyclase | - |
Rhodobacter capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Cobalamin | dependent on, ligated via Asp248, Glu249, Leu29, Thr71, Val97 | Rhodobacter capsulatus | |
additional information | membrane-localized BchE requires an additional, heat-sensitive cytosolic cobalamin component for activity, BchE catalysis is not sustained in chimeric experiments when a cytosolic extract from Escherichia coli is used as a substitute | Rhodobacter capsulatus | |
S-adenosyl-L-methionine | dependent on, ligated via Phe210, Glu308 and Lys320 | Rhodobacter capsulatus | |
[4Fe-4S] cluster | ligated viaCys204, Cys208, Cys211 | Rhodobacter capsulatus |
General Information | Comment | Organism |
---|---|---|
physiological function | during bacteriochlorophyll a biosynthesis, the oxygen-independent conversion of Mg-protoporphyrin IX monomethyl ester (Mg-PME) to protochlorophyllide (Pchlide) is catalyzed by the anaerobic Mg-PME cyclase termed BchE, via an unusual radical S-adenosylmethionine (SAM) and cobalamin-dependent BchE catalysis. Membrane-localized BchE requires an additional, heat-sensitive cytosolic component for activity | Rhodobacter capsulatus |