BRENDA - Enzyme Database
show all sequences of 1.21.98.3

Identification of the chlE gene encoding oxygen-independent Mg-protoporphyrin IX monomethyl ester cyclase in cyanobacteria

Yamanashi, K.; Minamizaki, K.; Fujita, Y.; Biochem. Biophys. Res. Commun. 463, 1328-1333 (2015)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene bchE, phylogenetic analysis
Rhodobacter capsulatus
gene Cyan7425_4778 or chlE, DNA and amino acid sequence determination and analysis, the chlE gene is located in a gene cluster, chlAII-ho2-hemN-chlE-desF, sequence comparisons and phylogenetic analysis, functional complementation of a bchE-lacking mutant of Rhodobacter capsulatus
Cyanothece sp. PCC 7425
gene Cyan7822_3999 or chlE, DNA and amino acid sequence determination and analysis, the chlE gene is located at some distance from the chlAII-ho2-hemN gene cluster, sequence comparisons and phylogenetic analysis, functional complementation of a bchE-lacking mutant of Rhodobacter capsulatus
Gloeothece verrucosa PCC 7822
Engineering
Protein Variants
Commentary
Organism
additional information
bchE genes from Cyanothece strains PCC 7425 and PCC 7822 restore the photosynthetic growth and bacteriochlorophyll production in the bchE-lacking mutant of Rhodobacter capsulatus
Rhodobacter capsulatus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O
Cyanothece sp. PCC 7425
-
3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
-
?
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O
Gloeothece verrucosa PCC 7822
-
3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
-
?
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O
Rhodobacter capsulatus
-
3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
-
?
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O
Rhodobacter capsulatus SB1003
-
3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Cyanothece sp. PCC 7425
B8HM76
-
-
Gloeothece verrucosa PCC 7822
E0U5T3
-
-
Rhodobacter capsulatus
P26168
-
-
Rhodobacter capsulatus SB1003
P26168
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O
-
744228
Cyanothece sp. PCC 7425
3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
-
-
?
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O
-
744228
Gloeothece verrucosa PCC 7822
3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
-
-
?
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O
-
744228
Rhodobacter capsulatus
3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
-
-
?
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O
-
744228
Rhodobacter capsulatus SB1003
3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
BChE
-
Rhodobacter capsulatus
chlE
-
Cyanothece sp. PCC 7425
chlE
-
Gloeothece verrucosa PCC 7822
Cyan7425_4778
-
Cyanothece sp. PCC 7425
Cyan7822_3999
-
Gloeothece verrucosa PCC 7822
MPE cyclase
-
Cyanothece sp. PCC 7425
MPE cyclase
-
Gloeothece verrucosa PCC 7822
MPE cyclase
-
Rhodobacter capsulatus
oxygen-independent Mg-protoporphyrin IX monomethyl ester cyclase
-
Cyanothece sp. PCC 7425
oxygen-independent Mg-protoporphyrin IX monomethyl ester cyclase
-
Gloeothece verrucosa PCC 7822
oxygen-independent Mg-protoporphyrin IX monomethyl ester cyclase
-
Rhodobacter capsulatus
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Cyanothece sp. PCC 7425
S-adenosyl-L-methionine
-
Gloeothece verrucosa PCC 7822
S-adenosyl-L-methionine
-
Rhodobacter capsulatus
Cloned(Commentary) (protein specific)
Commentary
Organism
gene bchE, phylogenetic analysis
Rhodobacter capsulatus
gene Cyan7425_4778 or chlE, DNA and amino acid sequence determination and analysis, the chlE gene is located in a gene cluster, chlAII-ho2-hemN-chlE-desF, sequence comparisons and phylogenetic analysis, functional complementation of a bchE-lacking mutant of Rhodobacter capsulatus
Cyanothece sp. PCC 7425
gene Cyan7822_3999 or chlE, DNA and amino acid sequence determination and analysis, the chlE gene is located at some distance from the chlAII-ho2-hemN gene cluster, sequence comparisons and phylogenetic analysis, functional complementation of a bchE-lacking mutant of Rhodobacter capsulatus
Gloeothece verrucosa PCC 7822
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Cyanothece sp. PCC 7425
S-adenosyl-L-methionine
-
Gloeothece verrucosa PCC 7822
S-adenosyl-L-methionine
-
Rhodobacter capsulatus
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
bchE genes from Cyanothece strains PCC 7425 and PCC 7822 restore the photosynthetic growth and bacteriochlorophyll production in the bchE-lacking mutant of Rhodobacter capsulatus
Rhodobacter capsulatus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O
Cyanothece sp. PCC 7425
-
3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
-
?
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O
Gloeothece verrucosa PCC 7822
-
3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
-
?
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O
Rhodobacter capsulatus
-
3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
-
?
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O
Rhodobacter capsulatus SB1003
-
3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O
-
744228
Cyanothece sp. PCC 7425
3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
-
-
?
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O
-
744228
Gloeothece verrucosa PCC 7822
3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
-
-
?
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O
-
744228
Rhodobacter capsulatus
3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
-
-
?
magnesium-protoporphyrin IX 13-monomethyl ester + 3 S-adenosyl-L-methionine + H2O
-
744228
Rhodobacter capsulatus SB1003
3,8-divinyl protochlorophyllide a + 3 5'-deoxyadenosine + 3 L-methionine
-
-
-
?
General Information
General Information
Commentary
Organism
evolution
BchE and ChlA/AcsF are mainly identified in anoxygenic photosynthetic bacteria and plants, respectively. Some photosynthetic bacteria have either both or one of the two enzymes. The phylogenetic relationships imply that both chlE/bchE and chlA/acsF genes had been the MPE cyclase genes inherited by a cyanobacterial common ancestor, and chlE has been later lost in several lineages. The chlA gene has persisted in all cyanobacterial lineages. A gene duplication of chlA to form chlAI and chlAII appears to have occurred in an early phase of evolution of cyanobacteria. Phylogenetic trees of ChlE/BchE and ChlA/AcsF, cyanobacteria and photosynthetic bacteria
Cyanothece sp. PCC 7425
evolution
BchE and ChlA/AcsF are mainly identified in anoxygenic photosynthetic bacteria and plants, respectively. Some photosynthetic bacteria have either both or one of the two enzymes. The phylogenetic relationships imply that both chlE/bchE and chlA/acsF genes had been the MPE cyclase genes inherited by a cyanobacterial common ancestor, and chlE has been later lost in several lineages. The chlA gene has persisted in all cyanobacterial lineages. A gene duplication of chlA to form chlAI and chlAII appears to have occurred in an early phase of evolution of cyanobacteria. Phylogenetic trees of ChlE/BchE and ChlA/AcsF, cyanobacteria and photosynthetic bacteria
Gloeothece verrucosa PCC 7822
evolution
BchE and ChlA/AcsF are mainly identified in anoxygenic photosynthetic bacteria and plants, respectively. Some photosynthetic bacteria have either both or one of the two enzymes. The phylogenetic relationships imply that both chlE/bchE and chlA/acsF genes had been the MPE cyclase genes inherited by a cyanobacterial common ancestor, and chlE has been later lost in several lineages. The chlA gene has persisted in all cyanobacterial lineages. A gene duplication of chlA to form chlAI and chlAII appears to have occurred in an early phase of evolution of cyanobacteria. Phylogenetic trees of ChlE/BchE and ChlA/AcsF, cyanobacteria and photosynthetic bacteria
Rhodobacter capsulatus
malfunction
bchE genes from Cyanothece strains PCC 7425 and PCC 7822 restore the photosynthetic growth and bacteriochlorophyll production in the bchE-lacking mutant of Rhodobacter capsulatus
Rhodobacter capsulatus
physiological function
expression of the bchE genes from Cyanothece strain PCC 7425 restores the photosynthetic growth and bacteriochlorophyll production in a bchE-lacking mutant of Rhodobacter capsulatus
Cyanothece sp. PCC 7425
physiological function
expression of the bchE genes from Cyanothece strain PCC 7822 restores the photosynthetic growth and bacteriochlorophyll production in a bchE-lacking mutant of Rhodobacter capsulatus
Gloeothece verrucosa PCC 7822
General Information (protein specific)
General Information
Commentary
Organism
evolution
BchE and ChlA/AcsF are mainly identified in anoxygenic photosynthetic bacteria and plants, respectively. Some photosynthetic bacteria have either both or one of the two enzymes. The phylogenetic relationships imply that both chlE/bchE and chlA/acsF genes had been the MPE cyclase genes inherited by a cyanobacterial common ancestor, and chlE has been later lost in several lineages. The chlA gene has persisted in all cyanobacterial lineages. A gene duplication of chlA to form chlAI and chlAII appears to have occurred in an early phase of evolution of cyanobacteria. Phylogenetic trees of ChlE/BchE and ChlA/AcsF, cyanobacteria and photosynthetic bacteria
Cyanothece sp. PCC 7425
evolution
BchE and ChlA/AcsF are mainly identified in anoxygenic photosynthetic bacteria and plants, respectively. Some photosynthetic bacteria have either both or one of the two enzymes. The phylogenetic relationships imply that both chlE/bchE and chlA/acsF genes had been the MPE cyclase genes inherited by a cyanobacterial common ancestor, and chlE has been later lost in several lineages. The chlA gene has persisted in all cyanobacterial lineages. A gene duplication of chlA to form chlAI and chlAII appears to have occurred in an early phase of evolution of cyanobacteria. Phylogenetic trees of ChlE/BchE and ChlA/AcsF, cyanobacteria and photosynthetic bacteria
Gloeothece verrucosa PCC 7822
evolution
BchE and ChlA/AcsF are mainly identified in anoxygenic photosynthetic bacteria and plants, respectively. Some photosynthetic bacteria have either both or one of the two enzymes. The phylogenetic relationships imply that both chlE/bchE and chlA/acsF genes had been the MPE cyclase genes inherited by a cyanobacterial common ancestor, and chlE has been later lost in several lineages. The chlA gene has persisted in all cyanobacterial lineages. A gene duplication of chlA to form chlAI and chlAII appears to have occurred in an early phase of evolution of cyanobacteria. Phylogenetic trees of ChlE/BchE and ChlA/AcsF, cyanobacteria and photosynthetic bacteria
Rhodobacter capsulatus
malfunction
bchE genes from Cyanothece strains PCC 7425 and PCC 7822 restore the photosynthetic growth and bacteriochlorophyll production in the bchE-lacking mutant of Rhodobacter capsulatus
Rhodobacter capsulatus
physiological function
expression of the bchE genes from Cyanothece strain PCC 7425 restores the photosynthetic growth and bacteriochlorophyll production in a bchE-lacking mutant of Rhodobacter capsulatus
Cyanothece sp. PCC 7425
physiological function
expression of the bchE genes from Cyanothece strain PCC 7822 restores the photosynthetic growth and bacteriochlorophyll production in a bchE-lacking mutant of Rhodobacter capsulatus
Gloeothece verrucosa PCC 7822
Other publictions for EC 1.21.98.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737606
Yamanashi
Identification of the chlE gen ...
Cyanothece sp. PCC 7425, Gloeothece verrucosa PCC 7822
Biochem. Biophys. Res. Commun.
463
1328-1333
2015
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7
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2
2
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744228
Yamanashi
Identification of the chlE ge ...
Cyanothece sp. PCC 7425, Gloeothece verrucosa PCC 7822, Rhodobacter capsulatus, Rhodobacter capsulatus SB1003
Biochem. Biophys. Res. Commun.
463
1328-1333
2015
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1
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9
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14
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4
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6
6
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726735
Boldareva-Nuianzina
Distribution and origin of oxy ...
Citromicrobium sp. CV44, Methylobacteriaceae bacterium RM11-8-1, Rhodobaca bogoriensis, Rhodobaca bogoriensis DSM 18756, Rhodobacterales bacterium chep-kr, Roseinatronobacter monicus, Roseinatronobacter sp. khil, Roseobacter sp., Roseobacter sp. B09, Roseobacter sp. Zun_kholvo
Appl. Environ. Microbiol.
79
2596-2604
2013
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10
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8
8
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689645
Gough
A new method for isolating phy ...
Rhodobacter capsulatus, Rhodobacter capsulatus ATCC BAA-309
Plant Physiol. Biochem.
45
932-936
2007
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1
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738567
Ouchane
Aerobic and anaerobic Mg-proto ...
Rubrivivax gelatinosus
J. Biol. Chem.
279
6385-6394
2004
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1
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3
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1
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1
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739563
Gough
Anaerobic chlorophyll isocycli ...
Rhodobacter capsulatus, Rhodobacter capsulatus ATCC BAA-309
Proc. Natl. Acad. Sci. USA
97
6908-6913
2000
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738495
Yang
Rhodobacter capsulatus genes i ...
Rhodobacter capsulatus, Rhodobacter capsulatus ATCC BAA-309
J. Bacteriol.
172
5001-5010
1990
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