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Literature summary for 1.21.98.3 extracted from

  • Gough, S.P.; Rzeznicka, K.; Peterson Wulff, R.; Francisco, J.d.; Hansson, A.; Jensen, P.E.; Hansson, M.
    A new method for isolating physiologically active Mg-protoporphyrin monomethyl ester, the substrate of the cyclase enzyme of the chlorophyll biosynthetic pathway (2007), Plant Physiol. Biochem., 45, 932-936.
    View publication on PubMed
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Application

Application Comment Organism
synthesis simplified method to prepare Mg-protoporphyrin monomethyl ester from freeze dried BchE mutant Rhodobacter capsulatus DB575 cells by extraction with acetone/H2O/25% NH3. Isolated Mg-protoporphyrin monomethyl ester can be identified by absorption and fluorescence spectroscopy, and its purity analyzed by HPLC. The extracted Mg-protoporphyrin monomethyl ester can be dried and redissolved in buffered DMSO to be used as substrate. Mg-protoporphyrin monomethyl ester is in addition a substrate to magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase, EC 1.13.14.81 Rhodobacter capsulatus

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus P26168
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Rhodobacter capsulatus ATCC BAA-309 P26168
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Synonyms

Synonyms Comment Organism
BChE
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 Rhodobacter capsulatus