BRENDA - Enzyme Database show
show all sequences of 1.21.98.1

In vitro reconstitution of the radical S-adenosylmethionine enzyme MqnC involved in the biosynthesis of futalosine-derived menaquinon

Cooper, L.E.; Fedoseyenko, D.; Abdelwahed, S.H.; Kim, S.H.; Dairi, T.; Begley, T.P.; Biochemistry 52, 4592-4594 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
recombinant plasmid encoding mqnC from Bacillus halodurans C-125 is introduced into Escherichia coli BL21(DE3)-T1R containing the Escherichia coli suf operon for coexpression of His6-MqnC with iron-sulfur cluster biogenesis proteins
Bacillus halodurans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
[4Fe-4S] cluster
4Fe-4S cluster-dependent enzyme
Bacillus halodurans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dehypoxanthine futalosine + S-adenosyl-L-methionine
Bacillus halodurans
-
cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine
-
-
?
dehypoxanthine futalosine + S-adenosyl-L-methionine
Bacillus halodurans C-125
-
cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus halodurans
Q9K864
-
-
Bacillus halodurans C-125
Q9K864
-
-
Purification (Commentary)
Commentary
Organism
anaerobic purification by nickel affinity chromatography
Bacillus halodurans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dehypoxanthine futalosine + S-adenosyl-L-methionine
-
724385
Bacillus halodurans
cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine
-
-
-
?
dehypoxanthine futalosine + S-adenosyl-L-methionine
dehypoxanthine futalosine i.e. 3-[3-[(2R,3S,4R)-3,4,5-trihydroxytetrahydrofuran-2-yl]propanoyl]benzoate
724385
Bacillus halodurans
cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine
cyclic dehypoxanthinyl futalosine i.e. (2R,3S,4R)-3,4,5-trihydroxy-4'-oxo-3',4,4',5-tetrahydro-2'H,3H-spiro[furan-2,1'-naphthalene]-6'-carboxylate
-
-
?
dehypoxanthine futalosine + S-adenosyl-L-methionine
-
724385
Bacillus halodurans C-125
cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine
-
-
-
?
dehypoxanthine futalosine + S-adenosyl-L-methionine
dehypoxanthine futalosine i.e. 3-[3-[(2R,3S,4R)-3,4,5-trihydroxytetrahydrofuran-2-yl]propanoyl]benzoate
724385
Bacillus halodurans C-125
cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine
cyclic dehypoxanthinyl futalosine i.e. (2R,3S,4R)-3,4,5-trihydroxy-4'-oxo-3',4,4',5-tetrahydro-2'H,3H-spiro[furan-2,1'-naphthalene]-6'-carboxylate
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Bacillus halodurans
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant plasmid encoding mqnC from Bacillus halodurans C-125 is introduced into Escherichia coli BL21(DE3)-T1R containing the Escherichia coli suf operon for coexpression of His6-MqnC with iron-sulfur cluster biogenesis proteins
Bacillus halodurans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
[4Fe-4S] cluster
4Fe-4S cluster-dependent enzyme
Bacillus halodurans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dehypoxanthine futalosine + S-adenosyl-L-methionine
Bacillus halodurans
-
cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine
-
-
?
dehypoxanthine futalosine + S-adenosyl-L-methionine
Bacillus halodurans C-125
-
cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
anaerobic purification by nickel affinity chromatography
Bacillus halodurans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dehypoxanthine futalosine + S-adenosyl-L-methionine
-
724385
Bacillus halodurans
cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine
-
-
-
?
dehypoxanthine futalosine + S-adenosyl-L-methionine
dehypoxanthine futalosine i.e. 3-[3-[(2R,3S,4R)-3,4,5-trihydroxytetrahydrofuran-2-yl]propanoyl]benzoate
724385
Bacillus halodurans
cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine
cyclic dehypoxanthinyl futalosine i.e. (2R,3S,4R)-3,4,5-trihydroxy-4'-oxo-3',4,4',5-tetrahydro-2'H,3H-spiro[furan-2,1'-naphthalene]-6'-carboxylate
-
-
?
dehypoxanthine futalosine + S-adenosyl-L-methionine
-
724385
Bacillus halodurans C-125
cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine
-
-
-
?
dehypoxanthine futalosine + S-adenosyl-L-methionine
dehypoxanthine futalosine i.e. 3-[3-[(2R,3S,4R)-3,4,5-trihydroxytetrahydrofuran-2-yl]propanoyl]benzoate
724385
Bacillus halodurans C-125
cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine
cyclic dehypoxanthinyl futalosine i.e. (2R,3S,4R)-3,4,5-trihydroxy-4'-oxo-3',4,4',5-tetrahydro-2'H,3H-spiro[furan-2,1'-naphthalene]-6'-carboxylate
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Bacillus halodurans
General Information
General Information
Commentary
Organism
physiological function
the enzyme is part of the futalosine pathway for menaquinone biosynthesis
Bacillus halodurans
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is part of the futalosine pathway for menaquinone biosynthesis
Bacillus halodurans
Other publictions for EC 1.21.98.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724385
Cooper
In vitro reconstitution of the ...
Bacillus halodurans, Bacillus halodurans C-125
Biochemistry
52
4592-4594
2013
-
-
1
-
-
-
-
-
-
1
-
2
-
2
-
-
1
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
728819
Yajima
-
Synthesis of (+-)-cyclic dehyp ...
Streptomyces coelicolor, Streptomyces coelicolor ATCC BAA-471
Tetrahedron Lett.
52
4934-4937
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
690059
Hiratsuka
An alternative menaquinone bio ...
Streptomyces coelicolor, Streptomyces coelicolor ATCC BAA-471
Science
321
1670-1673
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-