Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.21.4.5 extracted from

  • Xun, L.; Topp, E.; Orser, C.
    Purification and characterization of a tetrachloro-p-hydroquinone reductive dehalogenase from a Flavobacterium sp (1992), J. Bacteriol., 174, 8003-8007 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.21.4.5

Metals/Ions

Metals/Ions Comment Organism Structure
additional information optimal activity at 0.1 mM total salts Sphingobium chlorophenolicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 non-denaturing PAGE Sphingobium chlorophenolicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,3,5,6-tetrachlorohydroquinone + 2 glutathione Sphingobium chlorophenolicum the enzyme is involved in degradation of pentachlorophenol 2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide
-
 ?
2,3,6-trichlorohydroquinone + 2 glutathione Sphingobium chlorophenolicum the enzyme is involved in degradation of pentachlorophenol 2,6-dichlorohydroquinone + Cl- + glutathione disulfide
-
 ?

Organism

Organism UniProt Comment Textmining
Sphingobium chlorophenolicum Q03520
-
-

Oxidation Stability

Oxidation Stability Organism
sensitive to oxygen, a loss of enzyme activity occurrs when DTT is not included in elution buffers during purification Sphingobium chlorophenolicum

Purification (Commentary)

Purification (Comment) Organism
-
 Sphingobium chlorophenolicum

Storage Stability

Storage Stability Organism
-20°C, no measurable loss of activity over a period of 1 month Sphingobium chlorophenolicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3,5,6-tetrachlorohydroquinone + 2 glutathione the enzyme is involved in degradation of pentachlorophenol Sphingobium chlorophenolicum 2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide
-
 ?
2,3,5,6-tetrachlorohydroquinone + 2 glutathione the enzyme converts tetrachlorohydroquinone to trichlorohydroquinone and trichlorohydroquinone to dichlorohydroquinone. The enzyme uses glutathione but not NADPH, NADH, dithiothreitol, or ascorbic acid as the reducing agent Sphingobium chlorophenolicum 2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide
-
 ?
2,3,6-trichlorohydroquinone + 2 glutathione the enzyme is involved in degradation of pentachlorophenol Sphingobium chlorophenolicum 2,6-dichlorohydroquinone + Cl- + glutathione disulfide
-
 ?
2,3,6-trichlorohydroquinone + 2 glutathione the enzyme converts tetrachlorohydroquinone to trichlorohydroquinone and trichlorohydroquinone to dichlorohydroquinone. The enzyme uses glutathione but not NADPH, NADH, dithiothreitol, or ascorbic acid as the reducing agent Sphingobium chlorophenolicum 2,6-dichlorohydroquinone + Cl- + glutathione disulfide
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 30000, SDS-PAGE Sphingobium chlorophenolicum

Synonyms

Synonyms Comment Organism
PCP hydroxylase
-
 Sphingobium chlorophenolicum
tetrachloro-p-hydroquinone reductive dehalogenase
-
 Sphingobium chlorophenolicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Sphingobium chlorophenolicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
 Sphingobium chlorophenolicum

General Information

General Information Comment Organism
metabolism the enzyme is involved in degradation of pentachlorophenol Sphingobium chlorophenolicum