Literature summary for 1.21.4.5 extracted from

McCarthy, D.L.; Navarrete, S.; Willett, W.S.; Babbitt, P.C.; Copley, S.D.
Exploration of the relationship between tetrachlorohydroquinone dehalogenase and the glutathione S-transferase superfamily (1996), Biochemistry, 35, 14634-14642 .

Search for more literature for 1.21.4.5

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli JM105	Sphingobium chlorophenolicum

Protein Variants

Protein Variants	Comment	Organism
C13S	the mutant converts tetrachlorohydroquinone to 2,3,5-trichloro-6-S-glutathionylhydroquinone and an unidentified isomer of dichloro-S-glutathionylhydroquinone rather than to dichlorohydroquinone	Sphingobium chlorophenolicum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2,3,5,6-tetrachlorohydroquinone + 2 glutathione	Sphingobium chlorophenolicum	the enzyme converts tetrachlorohydroquinone to trichlorohydroquinone and trichlorohydroquinone to dichlorohydroquinone. The enzyme is involved in degradation of pentachlorophenol, a widely used wood preservative	2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide	-	?
2,3,6-trichlorohydroquinone + 2 glutathione	Sphingobium chlorophenolicum	the enzyme converts tetrachlorohydroquinone to trichlorohydroquinone and trichlorohydroquinone to dichlorohydroquinone. The enzyme is involved in degradation of pentachlorophenol, a widely used wood preservative	2,6-dichlorohydroquinone + Cl- + glutathione disulfide	-	?

Organism

Organism	UniProt	Comment	Textmining
Sphingobium chlorophenolicum	Q03520	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Sphingobium chlorophenolicum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,3,5,6-tetrachlorohydroquinone + 2 glutathione	the enzyme converts tetrachlorohydroquinone to trichlorohydroquinone and trichlorohydroquinone to dichlorohydroquinone. The enzyme is involved in degradation of pentachlorophenol, a widely used wood preservative	Sphingobium chlorophenolicum	2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide	-	?
2,3,5,6-tetrachlorohydroquinone + 2 glutathione	the enzyme converts tetrachlorohydroquinone to trichlorohydroquinone and trichlorohydroquinone to dichlorohydroquinone. In addition to trichlorohydroquinone and dichlorohydroquinone, the enzyme also produces substantial amounts of 2,3,5-trichloro-6-S-glutathionylhydroquinone and an unidentified isomer of dichloro-S-glutathionylhydroquinone. Treatment of the purified enzyme with dithiothreitol or use of freshly prepared crude extracts dramatically decreases the formation of S-glutathionylhydroquinone and the unidentified isomer of dichloro-S-glutathionylhydroquinone. 2,3,5-Trichloro-6-S-glutathionylhydroquinone and an unidentified isomer of dichloro-S-glutathionylhydroquinone are produced by enzyme that has undergone some type of oxidative damage and are therefore not physiologically relevant products. The fact that the oxidative damage can be repaired by DTT suggests that a cysteine or methionine residue may be involved	Sphingobium chlorophenolicum	2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide	-	?
2,3,6-trichlorohydroquinone + 2 glutathione	the enzyme converts tetrachlorohydroquinone to trichlorohydroquinone and trichlorohydroquinone to dichlorohydroquinone. The enzyme is involved in degradation of pentachlorophenol, a widely used wood preservative	Sphingobium chlorophenolicum	2,6-dichlorohydroquinone + Cl- + glutathione disulfide	-	?
2,3,6-trichlorohydroquinone + 2 glutathione	the enzyme converts tetrachlorohydroquinone to trichlorohydroquinone and trichlorohydroquinone to dichlorohydroquinone	Sphingobium chlorophenolicum	2,6-dichlorohydroquinone + Cl- + glutathione disulfide	-	?

Synonyms

Synonyms	Comment	Organism
pcpC	-	Sphingobium chlorophenolicum
TCHQ dehalogenase	-	Sphingobium chlorophenolicum
tetrachlorohydroquinone dehalogenase	-	Sphingobium chlorophenolicum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Sphingobium chlorophenolicum

General Information

General Information	Comment	Organism
metabolism	the enzyme is involved in degradation of pentachlorophenol, a widely used wood preservative	Sphingobium chlorophenolicum

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Release 2023.1 (January 2023)